An alternative approach to the detection of lignin: a note on the application of ELISA using polyclonal antibodies
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Abstract
A specific detection method for lignin has been developed using polyclonal antibodies raised against BSA coupled lignin. This method was found to be highly sensitive and linear in enzyme linked immunosorbant assay (ELISA) within the coating lignin concentration range of 0.01 μg/ml to 1 μg/ml. The interference in the detection due to the presence of lignin breakdown products and other coexisting biopolymers could be eliminated at an antiserum dilution of 1:25600.
Keywords
Enzyme Lignin Detection Method Polyclonal Antibody Immunosorbant Assay
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© Springer-Verlag Berlin Heidelberg 2000