Advertisement

Bioprocess Engineering

, Volume 22, Issue 1, pp 19–22 | Cite as

Purification of lysozyme by affinity-based reversed micellar two-phase extraction

  • Y. Sun
  • S. Bai
  • L. Gu
  • S. Furusaki
Article

Abstract

A dye-affinity reversed micellar system was used for lysozyme purification from a crude solution of chicken egg white. The dye-affinity reversed micelles consisted of Cibacron Blue F-3GA (CB; 0.1 mM) modified lecithin (50 g/l) in n-hexane. Starting with a crude egg white solution containing lysozyme of 0.0381 mg/mg protein, lysozyme purity was increased by 16 to 20 times, reached 0.62 to 0.76 mg/mg protein. The affinity micellar system was recycled and used three times. Addition of polyoxyethylene (20) sorbitan trioleate (Tween 85) as a cosurfactant could increase the capacity of the affinity-based reversed micelles. A lysozyme recovery yield of over 70% was obtained at a forward aqueous phase pH of 9.16 using the reversed micelles additionally containing 20 g/l of Tween 85.

Keywords

Purification Aqueous Phase Lysozyme Lecithin Reversed Micelle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Y. Sun
    • 1
  • S. Bai
    • 1
  • L. Gu
    • 1
  • S. Furusaki
    • 2
  1. 1.Department of Biochemical Engineering, Tianjin University, Tianjin 300072, People's Republic of ChinaCN
  2. 2.Department of Chemical Systems and Engineering, Kyushu University, Fukuoka 812-8581, JapanJP

Personalised recommendations