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Bioprocess Engineering

, Volume 19, Issue 1, pp 61–65 | Cite as

Screening and selection of media components for lactic acid production using Plackett–Burman design

  • S. Krishnan
  • S. G. Prapulla
  • D. Rajalakshmi
  • M. C. Misra
  • N. G. Karanth
Article

Abstract

Plackett–Burman design was used to efficiently select important media components influencing lactic acid production in a two step screening procedure. A total of 36 screening experiments were conducted for studying the effect of various media components such as carbon and nitrogen (simple and complex) sources, minerals/buffering agents and a specific inducer for the production of lactic acid by Lactobacillus plantarum NCIM 2084. The eleven ingredients chosen after the first screening experiments were further screened by a Plackett-Burman design consisting of 12 experiments. Liquefied starch, wheat bran extract, ammonium nitrate, manganese sulphate and sodium acetate were chosen as promising ingredients for further optimisation studies. The highest yield of 41.9 g/l of lactic acid was obtained at the end of 24 hours of fermentation which corresponded to 90% conversion, on the basis of sugar supplied.

Keywords

Fermentation Lactic Acid Lactobacillus Bran Sodium Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • S. Krishnan
    • 1
  • S. G. Prapulla
    • 1
  • D. Rajalakshmi
    • 2
  • M. C. Misra
    • 1
  • N. G. Karanth
    • 1
  1. 1.Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore 570 013, IndiaIN
  2. 2.Sensory Sciences Department, Central Food Technological Research Institute, Mysore 570 013, IndiaIN

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