Bioprocess Engineering

, Volume 17, Issue 6, pp 383–386

Ethanol production by Kluyveromyces marxianus IMB3 during growth on straw-supplemented whiskey distillery spent wash at 45 °C

  • N. Barron
  • H. Mulholland
  • M. Boyle
  • A. P. McHale
Article

DOI: 10.1007/PL00008971

Cite this article as:
Barron, N., Mulholland, H., Boyle, M. et al. Bioprocess Engineering (1997) 17: 383. doi:10.1007/PL00008971

Abstract

The thermotolerant, ethanol-producing yeast strain, Kluyveromyces marxianus IMB3 was grown on media consisting of straw-supplemented distillery spent wash from The Old Bushmill's Distillery Co. Ltd., Bushmills, Co Antrim, Northern Ireland. Media were supplemented with cellulase activity and fermentations were carried out at 45 °C. When pulverized straw was used as substrate in this system at concentrations of 2, 4 and 6% (w/v), ethanol concentrations increased to maxima of 1.45, 2.2 and 3 g/l, respectively. Based on straw containing a maximum of 40% cellulose, these ethanol concentrations accounted for 36, 27 and 24% of the maximum theoretical yield, respectively. When the straw was pre-treated with NaOH and used in the spent wash containing system at concentrations of 2, 4 and 6% (w/v) ethanol, concentrations increased to maxima of 3, 6.2 and 10.5 g/l, respectively and these accounted for 75, 76 and 86% of the maximum theoretical yield. When these results are compared with previously published data relating to the use of straw in laboratory-based media, they suggest that whiskey distillery spent wash may provide an adequate medium for supplementation with complex carbohydrate and subsequent ethanol production in simultaneous saccharification and fermentation processes.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • N. Barron
    • 1
  • H. Mulholland
    • 1
  • M. Boyle
    • 1
  • A. P. McHale
    • 1
  1. 1.Biotechnology Research Group, School of Applied Biological and Chemical Sciences, University of Ulster, Coleraine, Co. Londonderry, BT52 1SA, Northern Ireland e-mail: ap.mchale@ulst.ac.ukIE

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