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Molecular and General Genetics MGG

, Volume 262, Issue 6, pp 921–930 | Cite as

The alm1 + gene from Schizosaccharomyces pombe encodes a coiled-coil protein that associates with the medial region during mitosis

  • M. Jiménez
  • T. Petit
  • C. Gancedo
  • C. Goday
ORIGINAL PAPER

Abstract

We have isolated a cDNA that encodes a 142-kDa protein by immunoscreening of a Schizosaccharomyces pombe expression library with a new antibody, mAb8, that reveals spindle poles and equatorial ring-like structures in several organisms. This cDNA encodes a putative protein which we termed Alm (for abnormal long morphology). The protein is predicted to be a coiled-coil protein, containing a central α-helical domain flanked by non-helical terminal domains. Immunofluorescence analysis showed that Alm1 is localized in the medial region of the cell from anaphase to the end of cytokinesis. Cells carrying an alm1::ura4 + disruption are viable and exhibit an elongated morphology. Homozygous alm1::ura4 + diploids sporulated normally but the spores did not germinate. Spores that have inherited the disruption allele from a heterozygous alm1 + / alm1::ura4 + diploid germinated but generated smaller colonies. We propose that Alm1 participates in the structural organization of the medial region in S. pombe.

Key wordsSchizosaccharomyces pombe Mitosis Medial region Coiled-coil protein 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. Jiménez
    • 1
  • T. Petit
    • 2
  • C. Gancedo
    • 2
  • C. Goday
    • 1
  1. 1.Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas. Velázquez 144, 28006 Madrid, Spain E-mail: Claragoday@cib.csic.es Tel.: +34-91-5611800; Fax: +34-91-5627518ES
  2. 2.Instituto de Investigaciones Biomédicas “Alberto Sols”, C.S.I.C-UAM, Arturo Duperier 4, 28029 Madrid, SpainES

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