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Medical Microbiology and Immunology

, Volume 184, Issue 4, pp 181–184 | Cite as

Characterization of Borrelia burgdorferi sensu lato strains isolated from Ixodes ricinus in Mecklenburg-Vorpommern, Germany

  • Heike Lottmann
  • Bettina Wilske
  • H. Herrmann
ORIGINAL INVESTIGATION
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Abstract

  Epidemiological studies in Mecklenburg-Vorpommern have shown a high prevalence of Borrelia burgdorferi-infected ticks. A total of 17 B. burgdorferi sensu lato strains were isolated from ticks and investigated by Western blots (immunoblot) with eight monoclonal antibodies against different epitopes of the outer surface protein A (OspA). Except for one, all strains could be classified using this system. The majority of strains belonged to the B. garinii-associated OspA serotypes 3, 5 and 6. Three isolates were classified as OspA serotype 2 (B. afzelii). B. burgdorferi sensu stricto strains (OspA serotype 1) as well as B. garinii-associated OspA serotype 4 were not present.

Key words  Borrelia burgdorferi sensu lato Outer surface protein A Serotypes Monoclonal antibodies 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Heike Lottmann
    • 1
  • Bettina Wilske
    • 2
  • H. Herrmann
    • 1
  1. 1.Institut für Medizinische Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Martin-Luther-Strasse 6, D-17487 Greifswald, Germany, Tel.: +49-3834/63516; Fax: +49-3834/63497DE
  2. 2.Max von Pettenkofer Institut für Hygiene und Medizinische Mikrobiologie, Universität München, Pettenkoferstrasse 9 a, D-80336 Munich, GermanyDE

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