Planta

, Volume 210, Issue 3, pp 439–445

The targeting and accumulation of ectopically expressed oleosin in non-seed tissues of Arabidopsis thaliana

  • Frédéric Beaudoin
  • Johnathan A. Napier
Article

DOI: 10.1007/PL00008152

Cite this article as:
Beaudoin, F. & Napier, J. Planta (2000) 210: 439. doi:10.1007/PL00008152

Abstract.

 Full-length and N-terminal deletions of a sunflower (Helianthus annuus L.) oleosin protein were expressed ectopically in transgenic Arabidopsis thaliana (L.) Heynh. Immunological detection of the sunflower protein revealed that it accumulated in a range of non-oil-storing tissues, including leaves, roots and petals. This accumulation was shown to result from deposition in the microsomal membrane fraction. Expression in oil-storing tissues (such as seeds) of oleosin N-terminal deletions revealed impaired transfer from the endoplasmic reticulum to the oil body. In non-oil-storing tissues, accumulation in the microsomal membrane fraction was progressively reduced by N-terminal deletion. These data confirm the role of the endomembrane system in the targeting of the oleosin and its intimate relationship with oil-body biogenesis.

Keywords:Arabidopsis (oleosin) Endoplasmic reticulum – Oleosin – Seed-specific gene expression – Transgenic Arabidopsis 

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Frédéric Beaudoin
    • 1
  • Johnathan A. Napier
    • 1
  1. 1.IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UKGB

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