Planta

, Volume 203, Supplement 1, pp S187–S197 | Cite as

Evaluation of the three-dimensional clinostat as a simulator of weightlessness

  • Takayuki Hoson
  • Seiichiro Kamisaka
  • Yoshio Masuda
  • Masamichi Yamashita
  • Brigitte Buchen
Multicellular plants Plant reproduction

Abstract.

Concerns regarding the reliability of slow- and fast-rotating uni-axial clinostats in simulating weightlessness have induced the construction of devices considered to simulate weightlessness more adequately. A new three-dimensional (3-D) clinostat equipped with two rotation axes placed at right angles has been constructed. In the clinostat, the rotation achieved with two motors is computer-controlled and monitored with encoders attached to the motors. By rotating plants three-dimensionally at random rates on the clinostat, their dynamic stimulation by gravity in every direction can be eliminated. Some of the vegetative growth phases of plants dependent on the gravity vector, such as morphogenesis, are shown to be influenced by rotation on the 3-D clinostat. The validity of 3-D clinostatting has been evaluated by comparing structural parameters of cress roots and Chara rhizoids obtained under real microgravity with those obtained after 3-D clinostatting. The parameters analyzed up to now (organization of the root cap, integrity and polarity of statocytes, dislocation of statoliths, amount of starch and ER) demonstrate that the 3-D clinostat is a valuable device for simulating weightlessness.

Key words: Automorphosis (root shoot) Chara (rhizoid) Clinostat (three-dimensional random rotation) Lepidium Gravitropism Weightlessness (simulated) 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Takayuki Hoson
    • 1
  • Seiichiro Kamisaka
    • 1
  • Yoshio Masuda
    • 2
  • Masamichi Yamashita
    • 3
  • Brigitte Buchen
    • 4
  1. 1.Department of Biology, Faculty of Science, Osaka City UniversitySumiyoshi-ku, OsakaJapan
  2. 2.Tezukayama CollegeNaraJapan
  3. 3.Institute of Space and Astronautical ScienceYoshinodai, SagamiharaJapan
  4. 4.Botanisches Institut, Rheinische Friedrich-Wilhelms-Universität BonnBonnGermany

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