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International Journal of Legal Medicine

, Volume 113, Issue 2, pp 98–101 | Cite as

Forensic identification of skeletal remains from members of Ernesto Che Guevara’s guerrillas in Bolivia based on DNA typing

  • R. Lleonart
  • E. Riego
  • M. V. Saínz de la Pena
  • K. Bacallao
  • F. Amaro
  • M. Santiesteban
  • M. Blanco
  • H. Currenti
  • A. Puentes
  • F. Rolo
  • L. Herrera
  • J. de la Fuente
Original article

Abstract

We report the positive identification of several members of the guerrillas led by Ernesto “Che” Guevara on the 1960 s in Bolivia by means of DNA fingerprinting. Successful DNA typing of both short tandem repeat loci and the hypervariable region of the human mitochondrial DNA was achieved after extracting total DNA from bones obtained from two burial sites. Given the size of the Cuban database for the STR allele frequencies, a conservative approach was followed to estimate the statistical significance of the genetic evidence. The estimated probabilities of paternity for the two cases in which the paternity logic was applied were higher than 99%. One case was analyzed using mitochondrial DNA and could not be excluded from the identity proposed by the forensic anthropology team. A fourth case was identified by exclusion, on the basis of the positive identification of the other remains, the historical and other anthropological evidence.

Key words Short tandem repeat DNA typing Polymerase chain reaction Paternity analysis 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • R. Lleonart
    • 1
  • E. Riego
    • 1
  • M. V. Saínz de la Pena
    • 2
  • K. Bacallao
    • 3
  • F. Amaro
    • 3
  • M. Santiesteban
    • 2
  • M. Blanco
    • 4
  • H. Currenti
    • 2
  • A. Puentes
    • 2
  • F. Rolo
    • 4
  • L. Herrera
    • 1
  • J. de la Fuente
    • 1
  1. 1.Mammalian Cell Genetics Division, Center for Genetic, Engineering and Biotechnology, P.O. Box 6162, Havana, Cuba e-mail: orgsup@cigb.edu.cu, Fax +537–218070CU
  2. 2.Laboratorio Central de Criminalística, Zulueta 465, Habana Vieja, Havana, CubaCU
  3. 3.Instituto de Medicina Legal, Apartado 10600, Havana, CubaCU
  4. 4.Laboratorio de Investigaciones sobre Sida, Havana, CubaCU

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