Journal of Plant Growth Regulation

, Volume 18, Issue 2, pp 49–53 | Cite as

Effect of Wild-Type and Mutant Plant Growth-Promoting Rhizobacteria on the Rooting of Mung Bean Cuttings

  • S.  Mayak
  • T.  Tirosh
  • B. R.  Glick

Abstract.

Mung bean cuttings were dipped in solutions of wild type and mutant forms of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 and then incubated for several days until roots formed. The bacteria P. putida GR12-2 and P. putida GR12-2/aux1 mutant do not produce detectable levels of the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, whereas P. putida GR12-2/acd36 is an ACC deaminase minus mutant. All bacteria produce the phytohormone indole-3-acetic acid (IAA), and P. putida GR12-2/aux1 overproduces it. Treatment of cuttings with the above-mentioned bacteria affected the rates of ethylene production in the cuttings in a way that can be explained by the combined effects of the activity of ACC deaminase localized in the bacteria and bacterial produced IAA. P. putida GR12-2 and P. putida GR12-2/acd36-treated cuttings had a significantly higher number of roots compared with cuttings rooted in water. In addition, the wild type influenced the development of longer roots. P. putida GR12-2/aux1 stimulated the highest rates of ethylene production but did not influence the number of roots. These results are consistent with the notion that ethylene is involved in the initiation and elongation of adventitious roots in mung bean cuttings.

Key Words. Ethylene—Plant growth-promoting rhizobacteria—Adventitious roots—ACC deaminase—Indole-3-acetic acid—Mung bean 

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Copyright information

© 1999 Springer-Verlag New York Inc.

Authors and Affiliations

  • S.  Mayak
    • 1
  • T.  Tirosh
    • 1
  • B. R.  Glick
    • 2
  1. 1.The Kennedy-Leigh Centre for Horticultural Research, Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, IsraelIL
  2. 2.Department of Biology, University of Waterloo, Waterloo,Ontario N2L 3G1, CanadaCA

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