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Current Microbiology

, Volume 38, Issue 3, pp 168–175 | Cite as

Adaptive Response to Cold Temperatures in Vibrio vulnificus

  • Patrick J.  Bryan
  • Robert J.  Steffan
  • Angelo  DePaola
  • John W.  Foster
  • Asim K.  Bej

Abstract.

The effectiveness of rapid chilling or freezing of oysters to reduce Vibrio vulnificus levels in shellfish may be compromised by product handling procedures that permit cold adaptation. When a V. vulnificus culture was shifted from 35°C to 6°C conditions, it underwent transition to a non-culturable state. Cells adapted to 15°C prior to change to 6°C condition, however, remain viable and culturable. In addition, cultures adapted to 15°C were able to survive better upon freezing at −78°C compared with cultures frozen directly from 35°C. Inhibition of protein synthesis by addition of chloramphenicol in a V. vulnificus culture immediately prior to the exposure to the adaptive temperature eliminated inducible cold tolerance. These results suggest that cold-adaptive “protective” proteins may enhance survival and tolerance at cold temperatures. In addition, removal of iron from the growth medium by adding 2,2′-Dipyridyl prior to cold adaptation decreased the viability by approximately 2 logarithm levels. This suggests that iron plays an important role in adaptation at cold temperatures. Analysis of total cellular proteins on an SDS polyacrylamide gel electrophoresis, labeled with 35S-methionine during exposure at 15°C, showed elevated expressions of a 6-kDa and a 40-kDa protein and decreased expression of an 80-kDa protein. These results suggest that, for V. vulnificus, survival and tolerance at cold temperatures could be due to the expression of cold-adaptive proteins other than previously documented major cold shock proteins such as CS7.4 and CsdA. In this study, for the first time we have shown that exposure to an intermediate cold temperature (15°C) causes a cold adaptive response, helping this pathogen remain in culturable state when exposed to a much colder temperature (6°C). This adaptive nature to cold temperatures could be important for shellfish industry efforts to reduce the risk of V. vulnificus infection from consuming raw oysters.

Keywords

Vibrio Cold Temperature Cold Tolerance Cold Shock Dipyridyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Patrick J.  Bryan
    • 1
  • Robert J.  Steffan
    • 2
  • Angelo  DePaola
    • 3
  • John W.  Foster
    • 4
  • Asim K.  Bej
    • 1
  1. 1.Department of Biology, University of Alabama at Birmingham, 1300 University Boulevard, Birmingham, AL 35294-1170, USA US
  2. 2.Envirogen, Inc., 4100 Quakerbridge Road, Lawrenceville, NJ 08648, USA US
  3. 3.U.S. Food and Drug Administration, Gulf Coast Seafood Laboratory, Dauphin Island, AL 36528, USA US
  4. 4.Department of Microbiology and Immunology, University of South Alabama, Mobile, AL 36688, USA US

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