Current Microbiology

, Volume 38, Issue 1, pp 27–32 | Cite as

Industrial Dye Decolorization by Laccases from Ligninolytic Fungi

  • Elizabeth  Rodríguez
  • Michael A.  Pickard
  • Rafael  Vazquez-Duhalt

Abstract.

White-rot fungi were studied for the decolorization of 23 industrial dyes. Laccase, manganese peroxidase, lignin peroxidase, and aryl alcohol oxidase activities were determined in crude extracts from solid-state cultures of 16 different fungal strains grown on whole oats. All Pleurotus ostreatus strains exhibited high laccase and manganese peroxidase activity, but highest laccase volumetric activity was found in Trametes hispida. Solid-state culture on whole oats showed higher laccase and manganese peroxidase activities compared with growth in a complex liquid medium. Only laccase activity correlated with the decolorization activity of the crude extracts. Two laccase isoenzymes from Trametes hispida were purified, and their decolorization activity was characterized.

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Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Elizabeth  Rodríguez
    • 1
  • Michael A.  Pickard
    • 1
  • Rafael  Vazquez-Duhalt
    • 1
  1. 1.Instituto de Biotecnología UNAM, Apartado Postal 510-3, Cuernavaca, Morelos, 62250 México MX

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