Journal of Molecular Evolution

, Volume 48, Issue 4, pp 421–426

Close Evolutionary Relatedness of α-Amylases from Archaea and Plants

  • Štefan  Janeček
  • Emmanuel  Lévêque
  • Abdel  Belarbi
  • Bernard  Haye

Abstract.

The amino acid sequences of 22 α-amylases from family 13 of glycosyl hydrolases were analyzed with the aim of revealing the evolutionary relationships between the archaeal α-amylases and their eubacterial and eukaryotic counterparts. Two evolutionary distance trees were constructed: (i) the first one based on the alignment of extracted best-conserved sequence regions (58 residues) comprising β2, β3, β4, β5, β7, and β8 strand segments of the catalytic (α/β)8-barrel and a short conserved stretch in domain B protruding out of the barrel in the β3 →α3 loop, and (ii) the second one based on the alignment of the substantial continuous part of the (α/β)8-barrel involving the entire domain B (consensus length: 386 residues). With regard to archaeal α-amylases, both trees compared brought, in fact, the same results; i.e., all family 13 α-amylases from domain Archaea were clustered with barley pI isozymes, which represent all plant α-amylases. The enzymes from Bacillus licheniformis and Escherichia coli, representing liquefying and cytoplasmic α-amylases, respectively, seem to be the further closest relatives to archaeal α-amylases. This evolutionary relatedness clearly reflects the discussed similarities in the amino acid sequences of these α-amylases, especially in the best-conserved sequence regions. Since the results for α-amylases belonging to all three domains (Eucarya, Eubacteria, Archaea) offered by both evolutionary trees are very similar, it is proposed that the investigated conserved sequence regions may indeed constitute the ``sequence fingerprints'' of a given α-amylase.

Key words:α-Amylase —Thermococcus hydrothermalis— Archaeons — Eubacteria — Eukaryotes — Conserved sequence regions — Evolutionary relationships 

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Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Štefan  Janeček
    • 1
  • Emmanuel  Lévêque
    • 2
  • Abdel  Belarbi
    • 2
  • Bernard  Haye
    • 3
  1. 1.Institute of Microbiology, Slovak Academy of Sciences, Štefánikova 3, SK-81434 Bratislava, SlovakiaCS
  2. 2.Laboratoire de Microbiologie Générale et Moléculaire, EUROPOL'AGRO, UFR Sciences de Reims, BP 1039, F-51687 Reims Cedex 2, FranceFR
  3. 3.Laboratoire de Biochimie, EUROPOL'AGRO, UFR Sciences de Reims, BP 1039, F-51687 Reims Cedex 2, FranceFR

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