Inflammation Research

, Volume 51, Issue 7, pp 332–341

Genomic-scale analysis of gene expression profiles in TNF-α treated human umbilical vein endothelial cells

  • J. Zhou
  • Y. Jin
  • Y. Gao
  • H. Wang
  • G. Hu
  • Y. Huang
  • Q. Chen
  • M. Feng
  • C. Wu

Abstract.

Objective and design: TNF-α is a potent proinflammatory cytokine that plays an important role in immunity and inflammation, and in the control of cell proliferation, differentiation and programmed cell death. However, it is known that TNF-α is also the founding member of a still growing family of cytokines with diverse bioregulative functions. Its detailed molecular mechanisms on endothelial activation and injury remain to be elucidated. This study was aimed at determining genomic-scale gene expression profiles in TNF-α treated human endothelial cells.¶Materials and methods: In this study cultured human umbilical vein endothelial cells (HUVECs) were stimulated with TNF-α (10 ng/ml) for 2 and 16 h, respectively, and the gene expression pattern was profiled using a cDNA array representing 14000 gene/cDNA clusters.¶Results: In total, 72 known human genes were identified the expression levels of which altered over 2-fold in response to TNF-α stimulation. Such alteration was confirmed for IL-8 and MCP-1, with an independent quantitative mRNA assay. It was observed that genes with related biological functions were often temporally co-regulated.¶Conclusions: These results indicate the transcriptional pathways mediated by TNF-α inside the HUVECs. Expression profiling in HUVECs responding to TNF-α stimulation should give an understanding of the molecular mechanisms involved in vascular inflammation.

Key words: TNF-α - HUVECs - cDNA array - Gene expression profile 

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Copyright information

© Birkhäuser Verlag, 2002

Authors and Affiliations

  • J. Zhou
    • 1
  • Y. Jin
    • 2
  • Y. Gao
    • 3
  • H. Wang
    • 1
  • G. Hu
    • 2
  • Y. Huang
    • 4
  • Q. Chen
    • 1
  • M. Feng
    • 1
  • C. Wu
    • 5
  1. 1.The National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China, Fax: ++86 10 62565689, e-mail: fengmf@panda.ioz.ac.cnCN
  2. 2.Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, ChinaCN
  3. 3.Department of Cell Biology, College of Life Sciences, Peking University, Beijing 100871, ChinaCN
  4. 4.Department of Immunology, Beijing Anzhen Hospital, Beijing 100029, ChinaCN
  5. 5.The National Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China, Fax: ++86 10 62756876, e-mail: wuch@pku.edu.cnCN

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