Advertisement

Journal of Rubber Research

, Volume 21, Issue 1, pp 62–72 | Cite as

Antagonistic Effects of Fungicolous Ascomycetous Cladobotryum Semicirculare on Rigidoporus Microporus White Root Disease in Rubber Trees (Hevea Brasiliensis) under in vitro and Nursery Experiments

  • Y. K. GohEmail author
  • N. F. Marzuki
  • Y. A. Liew
  • Y. K. Goh
  • K. J. Goh
Article

Abstract

White root disease caused by the pathogenic Rigidoporus microporus (Sw.) Overeem, in rubber trees (Hevea brasiliensis Muell. Arg.) could limit latex production by reducing the number of productive rubber trees. Fungicolous ascomycetous Cladobotryum semicirculare G.R.W. Arnold, R. Kirschner & Chee J. Chen, was assessed for its capability of suppressing Rigidoporus microporus mycelia growth in vitro and its efficacy in reducing white root disease of rubber seedlings. Mycelia growth of Rigidoporus microporus was inhibited by Cladobotryum semicirculare mycelia (direct antagonism) with 79% inhibition at nine days-after-inoculation through a dual — culture assay and heat stable antifungal Cladobotryum semicirculare filtrates (exudates sterilised through either filter-sterilisation or autoclaving — antibiosis) followed by the poison agar test. Cladobotryum semicirculare was also found to reduce the regeneration of Rigidoporus microporus mycelia. Area under the disease progress curve, calculated using disease severity of white root disease, were reduced by 47 to 50% through application of Cladobotryum semicirculare inoculant on two separate scion-rootstocks (PB 350 — RRIM 2025 and PB 347 — RRIM 2025) combinations of rubber clones, respectively. There was no scion-rootstock interaction observed.

Keywords

Antifungal ascomycetes basidiomycetes disease severity dual-culture fungal extract hypomyces rhizomorph 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    ALLEN, P.W. and CRONIN, M.E. (1994) Analysis of the 1993/1994 IRRDB Survey on Severity of Diseases of Hevea. Cochin, India, IRRDB Symposium on Diseases of Hevea.Google Scholar
  2. 2.
    CHEE, K.H. (1990) Present Status of Rubber Diseases and Their Control. Rev. Plant Pathol, 69(7), 423–430.Google Scholar
  3. 3.
    JAYASINGHE, C.K. (1999) Pests and Diseases of Hevea Rubber and Their Geographical Distribution. Bull. Rubber Res. Inst. Sri Lanka, 40, 1–8.Google Scholar
  4. 4.
    MOHAMMED, C.L., RIMBAWANTO, A. and PAGE, D.E. (2014) Management of Basidiomycete Root — and Stem — Rot Diseases in Oil Palm, Rubber and Tropical Hardwood Plantation Crops. Forest Pathol, 44(6), 428–446.CrossRefGoogle Scholar
  5. 5.
    NANDRIS, D., CHADOEUF, J., PIERRAT, J.C., JOANNES, H., GEIGER, J.P. and NICOLE, M. (1996) Modelling Rubber-Tree Root Diseases, Simulations of Various Inoculum Rates and Methods of Control. Eur. J. Forest Pathol., 26(1), 25–44.CrossRefGoogle Scholar
  6. 6.
    SOEKIRMAN, P. and BUDI, S. (2009) Integrated Disease Management of White Root Disease on Hevea Rubber using Trichoderma and Antagonistic Plants. Getas Research Center. Indonesian Rubber Research Institute. In: Disease Management Strategies in Plantations, Jogjakarta, AusAID sponsored workshop, 4 -8 May.Google Scholar
  7. 7.
    CHAN, W.H., WONG, C.P. and WONG, C.C. (1991) Control of White Root Disease in Immature Rubber with Three Systematic Fungicides. The Planter, 67(783), 251–265.Google Scholar
  8. 8.
    JAYASURIYA, K.E. and THENNAKOON, B.I. (2007) Biological Control of Rigidoporus Microporus, The Cause of White Root Disease in Rubber. Cey. J. Sci. (Bio. Sci.), 36(1), 9–16.Google Scholar
  9. 9.
    KAEWCHAI, S. and SOYTONG, K. (2010) Application of Biofungicides Against Rigidoporus Microporus Causing White Root Disease of Rubber Trees. J. Agric. Technol., 6(2), 349–363.Google Scholar
  10. 10.
    GO, W.Z., H’NPG, P.S., WONG, M.Y., TAN, G.H., LUQMAN CHUAH, A., SALMIAH, U., TOCZYŁOWSKA-MAMIŃSKA, R., SONI, O., WONG, W.Z., CHIN, K.L. and CHAI, E.W. (2015) Occurrence and Characterisation of Mycoflora in Soil of Different Health Conditions Associated with White Root Disease in Malaysia Rubber Plantation. J. Rubb. Res., 18(3), 159–170.Google Scholar
  11. 11.
    MARZUKI, N.F., GOH, Y.K., TUNG, H.J., GOH, Y.K. and GOH, K.J. (2015) Evaluation on the Cultural Characteristics and Antagonistic Activities of Cladobotryum Semicirculare Against Ganoderma Boninense in Vitro. J. Oil Palm Res., 27(4), 326–338.Google Scholar
  12. 12.
    ROGERSON, C.T. and SAMUELS, G.J. (1993) Polyporicolous Species of Hypomyces. Mycologia, 85(2), 231–272.CrossRefGoogle Scholar
  13. 13.
    WHITE, T.J., BRUNS, T., LEE, S. and TAYLOR, J. (1990) Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics, In: INNIS, M.A., GELFAND, D.H., SNINSKY, J.J. and WHITE, T.J. (Eds.) PCR Protocols: A guide to Methods and Applications. New York: Academic Press, 315–322.Google Scholar
  14. 14.
    OGHENEKARO, A.O., MIETTINEN, O., OMORUSI, V.I., EVUEH, G.A., FARID, M.A., GAZIS, R. and ASIEGBU, F.O. (2014) Molecular Phylogeny of Rigidoporus Microporus Isolates Associated with White Rot Disease of Rubber Trees (Hevea Brasiliensis). Fungal Biol, 118(5 — 6), 495–506.CrossRefGoogle Scholar
  15. 15.
    TAMURA, K., STECHER, G., PETERSON, D., FILIPSKI, A. and KUMAR, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol., 30(12), 2725–2729.CrossRefGoogle Scholar
  16. 16.
    GOH, Y.K., MARZUKI, N.F., GOH, T.K., TAN, S.Y., GOH, Y.K. and GOH, K.J. (2016) Mycoparasitic Scytalidium Parasiticum as a Potential Biocontrol Agent Against Ganoderma Boninense Basal Stem Rot in Oil Palm. Biocontrol Sci. Technol., 26(10), 1352–1365.CrossRefGoogle Scholar
  17. 17.
    SUNDRAM, S. (2013) First Report: Isolation of Endophytic Trichoderma from Oil Palm (Elaeis Guineensis Jacq.) and their In Vitro Antagonistic Assessment on Ganoderma Boninense. J. Oil Palm Res., 25(3), 368–372.Google Scholar
  18. 18.
    KOK, S.M., GOH, Y.K., TUNG, H.J., GOH, K.J., GOH, Y.K. and WONG, W.C. (2013) In Vitro Growth of Ganoderma Boninense Isolates on Novel Palm Extract Medium and Virulence on Oil Palm Elaeis Guineensis) Seedlings. Malays. J. Microbiol, 9(1), 33–42.Google Scholar
  19. 19.
    SHANER, G. and FINNER, R.W. (1977) The Effect of Nitrogen Fertilisation on the Expression of Slow-Mildewing Resistance in Knox Wheat. Phytopathol., 77, 1051–1056.CrossRefGoogle Scholar

Copyright information

© The Malaysian Rubber Board 2018

Authors and Affiliations

  • Y. K. Goh
    • 1
    Email author
  • N. F. Marzuki
    • 1
  • Y. A. Liew
    • 1
  • Y. K. Goh
    • 1
  • K. J. Goh
    • 1
  1. 1.Advanced Agriecological Research Sdn BhdPetaling Jaya, Selangor Darul EhsanMalaysia

Personalised recommendations