Advertisement

The Journal of Physiological Sciences

, Volume 65, Supplement 2, pp S23–S28 | Cite as

Antinociceptive effects of Rhus coriaria L. extract in male rats

  • Saeed Mohammadi
  • Mohammad ZareiEmail author
  • Mohammad Mahdi Zarei
Original Paper
First Online:

Abstract

It is well known that the tendency toward the medicinal plants is increasing in recent years. They have low side-effects and high varieties of efficient components. This study was designed to investigate the analgesic effect of hydro alcoholic leaf extract of Rhus coriaria (HRCLE) in a rat model. For this purpose, 42 adult male rats were divided into 7 groups: control, HRCLE (80, 100 and 300 mg/kg, i.p.), morphine (1 mg/kg, i.p.), aspirin (1 mg/kg, i.p.), and HRCLE 300 mg/kg plus naloxone (1 mg/kg, i.p.). The analgesic effects of HRCLE were assessed with writhing, tail flick and formalin tests. The data were compared with control by one-way ANOVA and Tukey post hoc test. All dose levels of HRCLE inhibited the number of contractions induced by acetic acid in the writhing test signfiicantly. None of the dose levels of HRCE have been showed antinociceptive activity in the formalin test except the dose of 100 mg/kg (at chronic phase) and the dose of 300 mg/kg (at chronic- acute phase). In the tail flick model, the highest effect was at the dose of 300 mg/kg of HRCLE (P < 0.01). Utilization of naloxone plus extract inhibited the antinociceptive effect of HRCLE. In this study, our findings suggest that analgesic effect for the HRCLE may be mediated via both peripheral and central mechanisms. The presence of flavonoids might be responsible for the antinociceptive activity of this plant.

Keywords

Pain Formaldehyde Rhus coriaria Medicinal plants Rat 
This is a preview of subscription content, log in to check access.

References

  1. 1.
    Baker MD, JN Wood (2001) Involvement of Na+ channels in pain pathways. Trends Pharmacol Sci 22(1): 27–31.CrossRefGoogle Scholar
  2. 2.
    Javan M, et al. (1997) Antinociceptive effects of Trigonella foenum-graecum leaves extract. J Ethnopharmacol 58(2): 125–129.CrossRefGoogle Scholar
  3. 3.
    Nafisy A (1997) A review of traditional medicine in iran. Isfahan University Publications Isfahan 8(11): 121.Google Scholar
  4. 4.
    Neto A, et al. (2005) Analgesic and anti-inflammatory activity of a crude root extract of Pfaffia glomerata (Spreng) Pedersen. J Ethnopharmacol 96(1): 87–91.CrossRefGoogle Scholar
  5. 5.
    Zarei M, et al. (2015) The Antinociceptive Effects of Hydroalcoholic Extract of Bryonia dioica in Male Rats.CrossRefGoogle Scholar
  6. 6.
    Mohammadi S, et al. (2015) In Vivo Antinociceptive Effects of Persian Shallot (Alliumhirtifolium) in Male Rat.Google Scholar
  7. 7.
    Kossah R, et al. (2009) Comparative study on the chemical composition of Syrian sumac (Rhus coriaria L.) and Chinese sumac (Rhus typhina L.) fruits. Pakistan Journal of Nutrition, 8(10): 1570–1574.CrossRefGoogle Scholar
  8. 8.
    Wetherilt H, M Pala (1994) Herbs and spices indigenous to Turkey, Amsterdam: Elsevier Science BV.Google Scholar
  9. 9.
    Andrade SF, et al. (2007) Anti-inflammatory and antinociceptive activities of extract, fractions and populnoic acid from bark wood of Austroplenckia populnea. J Ethnopharmacol 109(3): 464–71.CrossRefGoogle Scholar
  10. 10.
    Duke JA (2002) Handbook of medicinal herbs: CRC press.Google Scholar
  11. 11.
    Brunke EJ, et al. (1993) The essential oil of Rhus coriaria L. fruits. Flavour and fragrance journal 8(4): 209–214.CrossRefGoogle Scholar
  12. 12.
    Nasar-Abbas S, AK Halkman (2004) Antimicrobial effect of water extract of sumac (Rhus coriaria L.) on the growth of some food borne bacteria including pathogens. International Journal of Food Microbiology 97(1): 63–69.CrossRefGoogle Scholar
  13. 13.
    Özcan M, H Haciseferogullari (2004) A condiment [sumac (Rhus coriaria L.) fruits]: some physicochemical properties. Bulgarian Journal of Plant Physiology 30(3-4): 74–84.Google Scholar
  14. 14.
    Kosar M, et al. (2007) Antioxidant activity and phenolic composition of sumac (Rhus coriaria L.) extracts. Food Chemistry 103(3): 952–959.CrossRefGoogle Scholar
  15. 15.
    Zimmermann M (1983) Ethical guidelines for investigations of experimental pain in conscious animals. Pain 16(2): 109–110.CrossRefGoogle Scholar
  16. 16.
    Collier H, et al. (1968) The abdominal constriction response and its suppression by analgesic drugs in the mouse. British journal of pharmacology and chemotherapy 32(2): 295–310.CrossRefGoogle Scholar
  17. 17.
    Gawade S (2012) Acetic acid induced painful endogenous infliction in writhing test on mice. Journal of Pharmacology and Pharmacotherapeutics 3(4): 348.CrossRefGoogle Scholar
  18. 18.
    D’amour FE, DL Smith (1941) A method for determining loss of pain sensation. Journal of Pharmacology and Experimental Therapeutics 72(1): 74–79.Google Scholar
  19. 19.
    Dubuisson D and SG Dennis (1978) The formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats. Pain 4: 161–174.CrossRefGoogle Scholar
  20. 20.
    Lorke D (1983) A new approach to practical acute toxicity testing. Archives of toxicology 54(4): 275–287.CrossRefGoogle Scholar
  21. 21.
    Russo S (2008) Integrated pain management: using omega 3 fatty acids in a naturopathic model. Techniques in Regional Anesthesia and Pain Management 12(2): 105–108.CrossRefGoogle Scholar
  22. 22.
    Fiorucci S, E. Antonelli, and A. Morelli (2001) Mechanism of non-steroidal anti-inflammatory drug-gastropathy. Digestive and Liver Disease 33: S35–S43.CrossRefGoogle Scholar
  23. 23.
    Negus SS, et al. (2006) Preclinical assessment of candidate analgesic drugs: recent advances and future challenges. Journal of Pharmacology and Experimental Therapeutics 319(2): 507–514.CrossRefGoogle Scholar
  24. 24.
    Koster R, M Anderson, E De Beer. Acetic acid-induced analgesic screening. (1959) Federation Proceedings.Google Scholar
  25. 25.
    Jensen TS, TL Yaksh (1986) I. Comparison of antinociceptive action of morphine in the periaqueductal gray, medial and paramedial medulla in rat. Brain research 363(1): 99–113.CrossRefGoogle Scholar
  26. 26.
    Tjølsen A, et al. (1992) The formalin test: an evaluation of the method. Pain 51(1): 5–17.CrossRefGoogle Scholar
  27. 27.
    Shibata M, et al. (1989) Modified formalin test: characteristic biphasic pain response. Pain 38(3): 347–352.CrossRefGoogle Scholar
  28. 28.
    Verma PR, et al. (2005) Antinociceptive activity of alcoholic extract of Hemidesmus indicus R. Br. in mice. J Ethnopharmacol 102(2): 298–301.CrossRefGoogle Scholar
  29. 29.
    Borras M, et al. (2004) fMRI measurement of CNS responses to naloxone infusion and subsequent mild noxious thermal stimuli in healthy volunteers. Journal of neurophysiology 91(6): 2723–2733.CrossRefGoogle Scholar
  30. 30.
    Hashemi SR, et al. (2008) Acute toxicity study and phytochemical screening of selected herbal aqueous extract in broiler chickens. International Journal of pharmacology 4(5): 352–360.CrossRefGoogle Scholar
  31. 31.
    Abu-Reidah IM, et al. (2015) HPLC-DAD-ESI-MS/MS screening of bioactive components from Rhus coriaria L.(Sumac) fruits. Food Chemistry 166: 179–191.CrossRefGoogle Scholar
  32. 32.
    Abu-Reidah IM, RM Jamous, MS Ali-Shtayeh (2014) Phytochemistry, Pharmacological Properties and Industrial Applications of Rhus coriaria L.(Sumac). Jordan Journal of Biological Sciences 7(4).Google Scholar
  33. 33.
    Di Carlo G, et al. (1999) Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life sciences 65(4): 337–353.CrossRefGoogle Scholar
  34. 34.
    Hashemi SR, H Davoodi (2011) Herbal plants and their derivatives as growth and health promoters in animal nutrition. Veterinary Research Communications 35(3): 169–180.CrossRefGoogle Scholar
  35. 35.
    Woodman OL, EC Chan (2004) Vascular and anti-oxidant actions of flavonols and flavones. Clinical and Experimental Pharmacology and Physiology 31(11): 786–790.CrossRefGoogle Scholar
  36. 36.
    Starec M, D Waitzova, J Elis (1988) Evaluation of the analgesic effect of RG-tannin using the “hot plate” and “tail flick” method in mice. Ceskoslovenská farmacie 37(7): 319.PubMedGoogle Scholar

Copyright information

© The Physiological Society of Japan and Springer Japan 2015

Authors and Affiliations

  • Saeed Mohammadi
    • 1
  • Mohammad Zarei
    • 2
    Email author
  • Mohammad Mahdi Zarei
    • 3
  1. 1.Department of Biology, Faculty of Basic SciencesIslamic Azad University of HamadanHamadanIran
  2. 2.Department of Physiology, Faculty of MedicineHamadan University of Medical SciencesHamadanIran
  3. 3.Faculty of Veterinary Medicine, Karaj BranchIslamic Azad UniversityKaraj, AlborzIran

Personalised recommendations

Cite article

Buy options