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Journal of Plant Diseases and Protection

, Volume 116, Issue 2, pp 49–54 | Cite as

Effect of fucoidan from the brown alga Fucus evanescens on the development of infection induced by potato virus X in Datura stramonium L. leaves

  • A. Reunov
  • L. Lapshina
  • V. Nagorskaya
  • T. Zvyagintseva
  • N. Shevchenko
Article

Abstract

We studied the influence of fucoidan from the brown alga Fucus evanescens on the development of infection induced by potato virus X (PVX) in Datura stramonium leaves. It is shown that 24 h after treatment of the leaves with fucoidan and following inoculation with PVX, the infectivity and amount of the virus in the leaves as well as the accumulation of viral particles in infected cells during early infection period were substantially less than those in the untreated control. Using ultrastructure-morphometric analysis, we established that the fucoidan treatment causes an increase in the protein-synthesizing capability of cells (nucleolus dimension and amount of both mitochondria and rough endoplasmic reticulum membranes increased). At the same time, the fucoidan treatment caused some stimulation of intracellular lytic processes that lead to destruction of virus particles and, therefore, may be considered as one of fucoidan-conditioned protective mechanisms limiting the virus accumulation in cells. Stimulation by fucoidan of the formation of PVX-specific laminated structures that may bind virus particles is another antiviral cell mechanism that limits reproduction and transportation of the virus.

Key words

antiviral activity Datura stramonium fucoidan Fucus evanescens potato virus X 

Effekt von Fukoidan aus der Braunalge Fucus evanescens auf den Infektionsverlauf von Kartoffelvirus X in Blättern von Datura stramonium L.

Zusammenfassung

Die Wirkung von Fukoidan aus der Braunalge Fucus evanescens auf den Infektionsverlauf von Kartoffelvirus X (PVX) in Blättern von Datura stramonium L. wurde untersucht. Während der frühen Infektionsphase, 24 Stunden nach der Behandlung der Blätter mit Fukoidan und der folgenden Inokulation mit PVX, waren das Infektionsvermögen, der Virusgehalt in den Blättern und die Zahl der Viruspartikel in den infizierten Mesophylzellen wesentlich geringer als in den Blättern der unbehandelten Kontrolle. Durch ultrastrukturell- morphometrische Analysen wurde festgestellt, dass die Behandlung mit Fukoidan die Fähigkeit der Zellen steigert, Proteine zu synthesieren (der Nukleolus und die Zahl der Mitochondrien und Membranen des rauhen endoplasmatischen Retikulums vergrößern sich). Zugleich stimuliert die Behandlung mit Fukoidan intrazelluläre lytischen Prozesse, die Viruspartikel abbauen, weshalb diese Prozesse als durch Fukoidan ausgelöste Abwehrmachanismen betrachtet werden können, die die Akkumulation der Viren beschränken. Die Bildung von PVX-spezifischen laminaren Strukturen durch Fukoidan, die möglicherweise die Viruspartikel binden können, ist ein weiterer vom Präparat induzierter antiviraler Zellschutzmechanismus, der die Reproduktion und Ausbreitung von PVX inhibiert.

Stichwörter

antivirale Aktivität Datura stramonium Fukoidan Fucus evanescens Kartoffelvirus X 

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Copyright information

© Deutsche Phythomedizinische Gesellschaft 2009

Authors and Affiliations

  • A. Reunov
    • 1
  • L. Lapshina
    • 1
  • V. Nagorskaya
    • 1
  • T. Zvyagintseva
    • 1
  • N. Shevchenko
    • 1
  1. 1.Pacific Institute of Bioorganic ChemistryFar East Branch of Russian Academy of SciencesVladivostokRussia

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