Journal of Plant Diseases and Protection

, Volume 114, Issue 2, pp 82–87 | Cite as

Quantitative analysis of the seasonal and tissue-specific expression of Cry1Ab in transgenic maize Mon810

  • H. T. Nguyen
  • J. A. JehleEmail author


The tissue-specific expression and seasonal abundance of Cry1Ab protein were determined in transgenic maize plants (Mon810, variety ‘Novelis’) from two field trials located near Bonn and Halle, Germany. A total of 1085 samples were analysed by using Double Antiserum-Enzyme Linked Immun-osorbent Assay (DAS-ELISA). The Cry1Ab contents of various plant tissues (root, stem, upper leaf, lower leaf, anther, pollen and kernel) were determined at four different growth stages (BBCH19, BBCH30, BBCH61 and BBCH83) collected in 2001, 2002 and 2003. Mon810 showed the highest Cry1Ab contents in the leaves (5.5–6.4 µg g–1 fresh weight [fw]) at BBCH83, whereas the lowest Cry1Ab contents were detected in the pollen (1–97 ng g–1 fw). Cry1Ab content of residual root stocks collected in the field nine months after harvest was 15–17 n g g–1 fw. This demonstrated that the Cry1Ab concentration in residual root stocks was reduced to about one-hundredth of the fresh roots. The monitoring of Cry1Ab expression showed that the Cry1Ab contents varied strongly between different plant individuals.

Key words

Cry1Ab expression ELISA European corn borer Mon810 transgenic maize 

Quantitative Analyse der saisonalen und gewebespezifischen Expression von Cry1Ab in transgenen Mon810-Mais


p ]Die gewebespezifische Expression und die saisonale Verbreitung des Cry1Ab-Proteins in transgenen Maispflanzen (Mon810, Sorte „Novelis“) wurde in zwei Feldversuchen bei Bonn und Halle untersucht. Insgesamt wurden 1085 Proben mit Hilfe des Double Antiserum-Enzyme Linked Immunosor-bent Assay (DAS-ELISA) untersucht. Der Cry1Ab-Gehalt verschiedener Pflanzengewebe (Wurzel, Stängel, oberes Blatt, unteres Blatt, Staubbeutel, Pollen und Korn) wurde in vier verschiedenen Entwicklungsstadien (BBCH19, BBCH30, BBCH61 and BBCH83) in den Jahren 2001, 2002 und 2003 bestimmt. Die Blätter von Mon810 zeigten den höchsten Cry1Ab-Gehalt (5.5–6.4 µg g–1 Frischgewicht [FG] bei BBCH83), während die Pollen mit 1–97 ng g–1 FG den geringsten Gehalt aufwiesen. Der Cry1Ab-Gehalt auf dem Feld verbliebener Wurzeln betrug neun Monate nach der Ernte 15–17 ng g–1 FG, also nur etwa ein Hundertstel des Gehalts frischer Maiswurzeln. Die Expression des Cry1Ab-Prote-ins variierte gravierend zwischen einzelnen Maispflanzen.


Cry1Ab-Expression ELISA Maiszünsler Mon810 transgener Mais 


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Copyright information

© Deutsche Phythomedizinische Gesellschaft 2007

Authors and Affiliations

  1. 1.Abt. Phytomedizin, Labor für Biotechnologischen PflanzenschutzDLR RheinpfalzNeustadt an der WeinstrasseGermany

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