Journal of Plant Diseases and Protection

, Volume 110, Issue 5, pp 409–418 | Cite as

Intra-species genomic groups in Fusarium semitectum and their correlation with origin and cultural characteristics

  • K. A. Abd-Elsalam
  • F. Schnieder
  • A. Asran-Amal
  • M. S. Khalil
  • J. A. Verreet
Article
First Online:
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Accepted:

Abstract

Fusarium semitectum, which occurs frequently among the fungal microflora associated with seedling disease of cotton, was examined using traditional mycological characteristics and molecular methods. The 29 Fusarium semitectum isolates from different cotton-producing areas and different host genotypes in Egypt could be divided undoubtedly into five groups based on cultural characteristics or Dna fingerprinting methods. The isolates showed a high level of variability in aerial mycelia growth, mycelia colour and radial mycelia growth (colony diameter) on potato dextrose agar. The intra-specific genomic relatedness of F. semitectum isolates was detected by Aflp and Rapd-Pcr fingerprinting methods and no particular difference was found between the results with the two fingerprint techniques.

Five genomic groups were obtained with both Rapd-Pcr and Aflp techniques. A good correlation of the five genomics groups and cultural characteristics were found, but no correlation between the results of the genetic analysis with the host genotype and geographic origin could be observed. The results indicate the utility of Rapd-Pcr and Aflp for identifying and monitoring intra-specific genetic variability for F. semitectum isolates.

Key words

cotton Fusarium pallidoroseum Fusarium semitectum RAPD AFLP 

Intraspezifische Gruppierung von Fusarium semitectum und ihre Korrelation mit der Herkunft und Koloniecharakteristika

Zusammenfassung

Fusarium semitectum, ein Pathogen, das häufig mit anderen Sämlingskrankheiten assoziiert ist, wurde mit traditionellen mykologischen und molekularen Techniken untersucht. Die 29 Isolate, die aus unterschiedlichen Anbauregionen der Baumwolle und Wirtsgenotypen isoliert wurden, konnten aufgrund ihrer morphologischen Charakteristika und der Ergebnisse aus den Fingerprints eindeutig in fünf Gruppen aufgeteilt werden. Die Isolate zeigten eine hohe Variabilität im Luftmycelwachstum, in der Pigmentierung und im radialem Myzelwachstum auf Pd-Agar.

Die intraspezifische, genomische Verwandtschaft der Isolate konnte mit der Aflp- und Rapd-Pcr Fingerprint-Methode nachgewiesen werden, wobei keine wesentlichen Unterschiede in den Ergebnissen der beiden Fingerprint-Techniken entdeckt wurden. Es konnten ebenfalls fünf genomische Gruppen abgeleitet werden. Allerdings konnte keine Korrelation zwischen den Ergebnissen der Fingerprint-Analyse und dem Wirtsgenotyp bzw. der Herkunft der Isolate hergestellt werden.

Die Ergebnisse zeigen, dass mit der Rapd-Pcr und Aflp-Technik eine Klassifizierung von F. semitectum möglich ist und die intraspezifische, genetische Variabilität bestimmt werden kann.

Stichwörter

Baumwolle Fusarium pallidoroseum Fusarium semitectum RAPD AFLP 
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Literature

  1. Appel, D. J., T. R. Gordon: Local and regional variation in populations of Fusarium oxysporum from agricultural field soils. — Phytopathology 84, 786–791, 1994.CrossRefGoogle Scholar
  2. Asstgbetse, K. B., D. Fernandez, M. P. Doubtos, J. P. Getger: Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by random ampilified polymorphic Dna (Rapd) analysis. — Phytopathology 84, 622–626, 1994.CrossRefGoogle Scholar
  3. Booth, C., B. C. Sutton: Fusarium pallidoroseum, the correct name for F. semitectum Auct. — Trans. Brit. Mycol. Soc. 83, 702–704, 1984.CrossRefGoogle Scholar
  4. Brown, J. K. M.: The choice of molecular marker methods for population genetic studies of plant pathogens. — New Phytol. 133, 183–195, 1996.CrossRefGoogle Scholar
  5. Chen-Shang, W., M. Hutqtn, W. Hut, S. W. Chen, M. Hq, H. Wang: Simultaneous identification of melon postharvest decay pathogens by the random amplified polymorphic Dna analysis with polymerase chain reaction. — Res. Prog. in Plant Prot. and Plant Nutr. 57, 109–114, 1999.Google Scholar
  6. Creste, S., A. Tulmann-Neto, A. Ftguetra: Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. — Pl. Mol. Biol. Rep. 19, 1–8, 2001.Google Scholar
  7. Donaldson, G. C., L. A. Ball, P. E. Axlrood, N. L. Glass: Primer sets developed to amplify conserved genes from filamentous ascomycetes are useful in differentiating Fusarium species associated with conifers. — Theor. Appl. Gen. 61, 1331–1340, 1995.Google Scholar
  8. El-Samawaty, A. M. A.: Studies on cotton root rot disease. — MSc. Thesis, Univ. Assiut, Egypt, 1999.Google Scholar
  9. Ellworth, D. L., K. D. Rittenhouse, R. L. Honeycut: Artifactual variation in randomly amplified polymorphic DNA banding patterns. — Biotechniques 14, 214–217, 1993.Google Scholar
  10. Feng, J. I. E., S. Wen, S. Leiyan, M. A. Cun, J. Feng, W. J. Sun, C. L. Y. Shi: RAPD analysis of physiologic races of Fusarium oxysporum f. sp. vasinfectum in China. — Mycosystema 19, 45–50, 2000.Google Scholar
  11. Gerlach W., H. Nirenberg: The genus Fusarium: A pictorial atlas. — Mitt. Biol. BundAnst. Ld. u. Forstwirtsch., Berlin 209, 1–406, 19&Google Scholar
  12. Gonzalez, M. M., E. Z. Rodriguez, J. L. Jacabo, F. Hernandez, J. Acosta, O. Martinez J. Simpson: Characterization of Mexican isolates of Colletotrichum lindemuthianum by using differential cultivars and molecular markers. — Phytopathology 88, 292–299, 1998.CrossRefPubMedGoogle Scholar
  13. Gordon, T. R., D. Okamoto: Variation within and between populations of Fusarium oxysporum based on vegetative compatibility and mitochondrial DNA. — Can. J. Bot. 70, 1211–1217, 1992.CrossRefGoogle Scholar
  14. Hillocks, R. J.: Cotton diseases. — CAB. International, Wallingford, United Kingdom, 1992.Google Scholar
  15. Janssen, P., R. Coopman, G. Huys, J. Swings, H. Bleeker, P. Vos, M. Zabeau, K. Kersters: Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. — Microbiol. 142, 1881–1893, 1996.CrossRefGoogle Scholar
  16. Kiprop, E. K., J. P. Baudoin, A. W. Mwang’Ombe, P. M. Kimani, G. Mergeai, A. Maquet: Characterization of Kenyan isolates of Fusarium udum from pigeonpea [Cajanus cajan (L.) Millsp.] by cultural characteristics, aggressiveness and AFLP analysis. — J. Phytopath. 150, 517–527, 2002.CrossRefGoogle Scholar
  17. Leissner, C. E. W., M. L. Niessen, R. F. Vogel: Use of the AFLP technique for the identification and discrimination of Fusarium graminearum. — Cereal Res. Commun. 25, 555–556, 1997.Google Scholar
  18. Majer, D., R. Mithen, B. Lewis, P. Vos, R. P. Oliver: The use of AFLP fingerprinting for the detection of genetic variation in fungi. — Mycol. Res. 100, 1107–1111, 1996.CrossRefGoogle Scholar
  19. Mayek-Perez, N., Z. Lopez-Castaneda, M. Gonzalez-Chavira, R. Garcia-Espinosa, J. Acosta-Gallegos, O. Martinez-Dela-Vega, J. Simpson: Variability of Mexican isolates of Macrophomina phaseolina based on pathogenesis and AFLP genotype. — Physiol. Mol. Pl. Pathol. 59, 257–264.Google Scholar
  20. Mantel, N.: The detection of disease clustering and a generalized regression approach. — Cancer Res. 27, 209–220, 1967.PubMedGoogle Scholar
  21. Miller, S. A.: Detecting propagules of plant pathogenic fungi. — Adv. Bot. Res. 23, 73–102, 1996.CrossRefGoogle Scholar
  22. Nei, M., W. H. Li: Mathematical model for studying genetic variation in terms of restriction endo-nucleases. — Proc. Natl. Acad. Sci. USA, 76, 5269–5273, 1979.CrossRefPubMedPubMedCentralGoogle Scholar
  23. O’Neill, N. R., B. A. Bailey, P. B. Van-Berkum: AFLP, a novel PCR-based DNA analysis technique, reveals level of genomic variation within species of Colletotrichum, Fusarium and Dendryphion. — In: 7th International Congress of Plant Pathology, Edinburgh, Scotland, 9–16 August, 1998.Google Scholar
  24. Palmateer, A., K. McLean, G. Morgan-Jones: Fungi involved in the seedling complex on Albama cotton. — Mag. Res. Ala. Agric. Exp. Stn. 48, 3, 2001.Google Scholar
  25. Pizzinatto, M. A., J. O. M. Menten: Pathogenicity of eight Fusarium spp. isolated from seeds to cotton seedlings. — Summa Phytopathol. 17, 124–134, 1991.Google Scholar
  26. Rayner, R. W.: A mycological colour chart. — Commonwealth Mycological Institute, Kew, UK, 1970.Google Scholar
  27. Roux, K. H.: Optimization and troubleshooting in PCR. — PCR Methods Appl. 4, 5185–5194, 1995.CrossRefGoogle Scholar
  28. Sambrock, J., Fritsch, E. F. & T. Maniatis: Molecular cloning: A laboratory manual (sec. Edition). Cold Spring Harbour Press, New York, U. S. A., 1989.Google Scholar
  29. Schnieder, F., G. Koch, C. Jung, J.-A. Verreet: Genotypic diversity of the wheat leaf blotch pathogen Mycosphaerella gramincola (anamorph Septoria tritici) in Germany. — Eur. J. Pl. Pathol. 107, 285–290, 2001.CrossRefGoogle Scholar
  30. Smith, S. N., J. E. Devay, H. W. Hsui, L.-H. Jen, W. H. Hsieh, H. J. Lee: Soil-borne populations of Fusarium oxysporum f. sp. vasinfectum, a cotton wilt fungus in California fields. — Mycologia 93, 737–743, 2001.CrossRefGoogle Scholar
  31. Sneath, P. H. A., R. R. Sokal: Numerical taxonomy. — Freeman and Company, San Francisco, 1973.Google Scholar
  32. Soleimany, M. J., G. A. Hedjaroude, J. Zad: Studies on pathogenicity of some seedborne Fusarium species on cotton seedling.— Iran J. Pl. Pathol. 29, 19–20, 1993.Google Scholar
  33. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van der Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper, M. Zabeau: Aflp: a new technique for Dna fingerprinting. — Nucleic Acids Res. 23, 4407–4424, 1995.PubMedGoogle Scholar
  34. Wang, G. C., Z. F. Gu, X. Lou, Q. M. Ye: Studies on the pathogens of Fusarium red rot of cotton. — Acta Phytopathol. Sin. 22, 211–215, 1992.Google Scholar
  35. Welsh, J., M. McClelland: Fingerprinting genomes using Pcr with arbitrary primers. — Nucleic Acids Res. 18, 7213–7218, 1990.CrossRefPubMedPubMedCentralGoogle Scholar
  36. Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, S. V. Tingey: Dna polymorphisms amplified by arbitrary primers are useful as genetic markers. — Nucleic Acids Res. 18, 6531–6535, 1990.CrossRefPubMedPubMedCentralGoogle Scholar
  37. Yli-Mattila, T., S. Paavanen, A. Hannukkala, P. Parikka, R. Tahvonen, R. Karjalainen: Iso- zyme and Rapd-Pcr analysis of Fusarium avenaceum strains from Finland. — Pl. Pathol. 45, 126–134, 1996.CrossRefGoogle Scholar
  38. Zabeau, M., P. Vos: Selective restriction fragment amplification: a general method for Dna fingerprinting. — European Patent Office, publication 0 534 858 A1, bulletin 93/13, 1993.Google Scholar
  39. Zhang, J. X., C. R. Howell, J. L. Starr, M. H. Wheeler, J. X. Zhang: Frequency of isolation and the pathogenicity of Fusarium species associated with roots of healthy cotton seedlings. — Mycol. Res. 100, 747–752, 1996.CrossRefGoogle Scholar
  40. Zhu, H., F. Qu, L. H. Zhu: Isolation of genomic Dnas from plants, fungi and bacteria using benzyl chloride. — Nucleic Acids Res. 2, 5279–5280, 1993.CrossRefGoogle Scholar

Copyright information

© Deutsche Phythomedizinische Gesellschaft 2003

Authors and Affiliations

  • K. A. Abd-Elsalam
    • 1
  • F. Schnieder
    • 1
  • A. Asran-Amal
    • 2
  • M. S. Khalil
    • 2
  • J. A. Verreet
    • 1
  1. 1.Institute of PhytopathologyChristian-Albrechts-University of KielKielGermany
  2. 2.Plant Pathology Research InstituteAgricultural Research CenterGizaEgypt

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