Imaging Subsurface Tissue Using Polarized Light - Numerical Results
- 27 Downloads
Abstract
Tissue images obtained at deeper depths lack significant contrast. To enhance the contrast of these images and to increase the visibility of subsurface tissues, a method is proposed. This technique is based on the principle that photons at longer wavelengths penetrate deeper than photons at shorter wavelengths. In this technique, images in the original and orthogonal polarized states are recorded with the source illumination in linear polarized state in two different wavelengths. Image subtraction of a fraction of the copolarized image from the perpendicular polarized state is done on the images obtained at the two wavelengths. The images obtained after the first processing are subjected to the next image subtraction where the lower wavelength image is subtracted from the higher wavelength image. Monte Carlo simulations show that image has marked contrast upto 3 optical depths.
Keywords
Optical Coherence Tomography Optical Depth Intensity Profile Orthogonal State Deep DepthPreview
Unable to display preview. Download preview PDF.
References
- 1.D. Huang, A. Swanson, C.P. Lin, J.S. Schuman, W.G. Stinson, W. Chang, M.R. Hee, T. Flotte, K. Gregory, C. Apuliafito and J. Fujimoto, Optical Coherence Tomography, Science, 254. 1178–1181 (1991).CrossRefADSGoogle Scholar
- 2.Johannes F. de Beer, Thomas E. Milner, Martin J.C. Van Gemert, and J. Stuart Nelson, Two dimensional birefringence imaging in biological tissue by polarization-sensitive optical coherence tomography, Optics letters, 22, 934–936 (1997).CrossRefADSGoogle Scholar
- 3.Andrew K. Dunn, Vincent P. Wallace, Mariah Coleno, Michale W. Bems, and Bruce J. Tromberg, Influence of optical properties on two - photon fluorescence imaging in turbid samples, Applied optics, 39, 1194–1201 (2000)CrossRefADSGoogle Scholar
- 4.W. Denk, J. H. Strickler and w.W. Webb, 2-photon laser scanning fluorescence microscopy, Science 248, 73–76, (1990).CrossRefADSGoogle Scholar
- 5.Barry R. Masters, Andres Kriete and Jorg Kukulies, Ultraviolet confocal fluorescence microscopy of the invitro cornea-redox metabolic imaging, Appl. Opt. 32, 592–596 (1993).CrossRefADSGoogle Scholar
- 6.S.L. Jacues, J. R Roman, K. Lee, Imaging superficial tissues with polarized light, Laser. Surg. Med 26, 119 (2000).CrossRefGoogle Scholar
- 7.P.C.Y. Chang, J.G. walker, K.I. Hopcraft, B. Ablitt, E. Jakeman, Polarization discrimination for active imaging in scattering media, Optics Communications, 159, 1–6 (1999).CrossRefADSGoogle Scholar
- 8.G. Turpin, J.G. Walker, P.C.Y. Chang, K.I. Hopcraft, B. Ablitt, E. Jakeman, The influence of particle size in active polarization imaging in scattering media, Optics Communication, 168, 325–335 (1999).CrossRefADSGoogle Scholar
- 9.S.G. Demos and R.R. Alfano, Optical polarization imaging, Applied Optics, 36, 150–155 (1997).CrossRefADSGoogle Scholar
- 10.S.G. Demos, H.B. Radousky and R.R. Alfani, Deep subsurface imaging in tissues using spectral and polarization filtering. Optics Express, 7, 23–28, (2000).CrossRefADSGoogle Scholar
- 11.Shuliang Jiao, Gang Yao and Lihong V. Wang, Depth resolved two dimensional Stokes vectors of backscattered light and Mueller matrices of biological tissue measured with OCT, Applied Optics, 39, 6318–6324 (2000).CrossRefADSGoogle Scholar
- 12.Craig F Bohren and Donald R Huffman, Absorption and Scattering of Light by small Particles, Wiley Interscience Publication, 1983.Google Scholar
- 13.John G. Walker, Peter C. Y. Chang and Keith I. Hopcraft, Visibility depth improvement in active polarization imaging in scattering media, Applied Optics, 39, 4933–4941 (2000)CrossRefADSGoogle Scholar
- 14.R.C. Gonsalez and R.E. Woods, Digital Image Processing, 3rd Edition, Addison Wesley, New York (1992).Google Scholar
- 15.Anil K. Jain, Fundamentals of Digital Image Processing, Eight Indian Edition, Prentice Hall of India (2002).Google Scholar