Journal of Endocrinological Investigation

, Volume 21, Issue 10, pp 668–672 | Cite as

Angiotensin II stimulates both aldosterone secretion and DNA synthesis via type 1 but not type 2 receptors in bovine adrenocortical cells

  • Akiyo Tanabe
  • M. Naruse
  • K. Arai
  • K. Naruse
  • T. Yoshimoto
  • T. Seki
  • T. Imaki
  • M. Kobayashi
  • H. Miyazaki
  • H. Demura
Article
First Online:
Accepted:

Abstract

Angiotensin II (Ang II) type 2 receptor (AT2) has been shown to counteract the type 1 receptor (AT1)-mediated biological actions of Ang II in the cardiovascular system. The biological significance of AT2 receptor in the adrenals however remains unknown. In the present study, we investigated the roles of ATI and AT2 receptor subtypes in the regulation of aldosterone secretion and DNA synthesis in bovine adrenocortical zona glomerulosa cells in vitro. Ang II (1 µmol/l)-stimulated aldosterone secretion was completely suppressed by AT1 antagonist CV-11974 but not affected by AT2 receptor antagonist PD-123319. Effects on DNA synthesis were investigated by determining the incorporation of BrdU into the nuclei of the cultured zona glomerulosa cells. Ang II (1 µmol/l)-stimulated DNA synthesis of the cells was also completely suppressed by CV-11974 but not by PD-123319. These results suggest that AT1 receptor but not AT2 receptor is the predominant receptor subtype which mediates the Ang II-stimulated aldosterone secretion and cell growth in bovine adrenocortical cells.

Key-words

Bovine adrenal cortex Angiotensin II AT1 receptor AT2 receptor aldosterone DNA synthesis zona glomerulosa cells 
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Copyright information

© Italian Society of Endocrinology (SIE) 1998

Authors and Affiliations

  • Akiyo Tanabe
    • 1
  • M. Naruse
    • 1
  • K. Arai
    • 1
  • K. Naruse
    • 1
  • T. Yoshimoto
    • 1
  • T. Seki
    • 1
  • T. Imaki
    • 1
  • M. Kobayashi
    • 2
  • H. Miyazaki
    • 3
  • H. Demura
    • 1
  1. 1.Department of MedicineTokyo Women’s Medical UniversityTokyoJapan
  2. 2.Department of PathologyTokyo Women’s Medical UniversityTokyoJapan
  3. 3.Institute of Applied Biochemistry, Gene Experiment CenterUniversity of TsukubaIbarakiJapan

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