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Journal of Endocrinological Investigation

, Volume 5, Issue 2, pp 101–106 | Cite as

Dose-related effects of γ-amino β-hydroxy butyric acid (GABOB) infusion on growth hormone secretion in normal women

  • Gian B. Melis
  • A. M. Paoletti
  • V. Mais
  • N. M. Mastrapasqua
  • F. Strigini
  • F. Fruzzetti
  • G. Guarnieri
  • M. Gambacciani
  • P. Fioretti
Article

Abstract

The effect of γ-aminoβ-hydroxy butyric acid (GABOB) infusion on GH secretion has been investigated in 3 groups of 6 normal women. Different doses (100 mg/min, 20 mg/min, 3.5 mg/min) of GABOB diluted with normal saline solution were infused iv over a 20 min period and compared with control tests performed using only saline solution. The infusions with GABOB at the doses of 100 mg/min and 20 mg/min were followed by a significant rise (p<0.01) in GH plasma levels, in comparison to control tests. The quantitative GH secretion after 100 mg/min GABOB infusion was significantly greater (p< 0.05) in comparison with quantitative GH secretion after 20 mg/min GABOB infusion. After 3.5 mg/min infusion, only 3 out of 6 subjects showed an increase in the hormone concentrations. The acute injection with sulpiride (5 mg iv, bolus), performed either before or after the start of GABOB infusion, failed to affect GH response to GABOB. These results show that GABOB infusion induce a dose-dependent rise in GH plasma levels in normal women. The dopaminergic system does not seem to be involved in this effect.

Key-words

Growth hormone GABA GABOB sulpiride dopamine 

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References

  1. 1.
    Nistri A., Costanti A. Pharmacological characterization of different types of GABA and glutamate receptors in vertebrates and invertebrates. Prog. Neurobiol. 13: 117, 1979.PubMedCrossRefGoogle Scholar
  2. 2.
    Jones M.T., Hillhouse E.W., Burden J. Effect of various putative neurotransmitters on the secretion of corticotrophin-releasing hormone from the rat hypothalamus vitro. A model of the neurotransmitters involved. J. Endocrinol. 69: 1, 1976.PubMedCrossRefGoogle Scholar
  3. 3.
    Vijayan E., McCann S.M. The effects of intraventricular injection of γ-aminobutyric acid (GABA) on prolactin and gonodotropin release in conscious female rats. Brain Res. 155: 35, 1978.PubMedCrossRefGoogle Scholar
  4. 4.
    Vijayan E., Mc Cann S.M. Effects of intraventricular injection of γ-aminobutyric acid (GABA) on plasma growth hormone and thyrotropin in conscious ovariectomized rats. Endocrinology 103: 1888, 1978.PubMedCrossRefGoogle Scholar
  5. 5.
    Takahara J., Yunoki S., Yakushiji W., Yamauchi J. Yamane Y., Ofuji T. Stimulatory effects of gamma-hydroxybutyric acid on growth hormone and prolactin release in humans. J. Clin. Endocrinol. Metab. 44: 1014, 1977.PubMedCrossRefGoogle Scholar
  6. 6.
    Tamminga C.A., Neophytides A., Chase T.N. Frohman L.A. Stimulation of prolactin and growth hormone secretion by muscimol, a γ-aminobutyric acid agonist. J. Clin. Endocrinol. Metab. 47: 1348, 1978.PubMedCrossRefGoogle Scholar
  7. 7.
    Takahara J. Yunoki S., Yakushiji W., Yamauchi J., Hosogi H., Ofuji T. Stimulatory effects of gamma-aminohydroxybutyric acid (GABOB) on growth hormone, prolactin and Cortisol release in man. Horm. Metab. Res. 12: 31, 1980.PubMedCrossRefGoogle Scholar
  8. 8.
    Cavagnini F., Invitti C., Di Landro A., Tenconi L., Maraschini G., Girotti G. Effects of a gamma aminobutyric acid (GABA) derivative, baclofen, on growth hormone and prolactin secretion in man. J. Clin. Endocrinol. Metab. 45: 579, 1977.PubMedCrossRefGoogle Scholar
  9. 9.
    Cavagnini F., Invitti C., Pinto M., Maraschini C., Di Landro A., Dubini A., Marelli A. Effect of acute and repeated administration of gamma aminobutyric acid (GABA) on growth hormone and prolactin secretion in man. Acta Endocrinol. (Kbh.) 93: 149, 1980.Google Scholar
  10. 10.
    Koulu M., Lammintausta R., Dahlström S. Stimulatory effect of acute baclofen administration on human growth hormone secretion. J. Clin. Endocrinol.Metab. 48: 1038, 1979.PubMedCrossRefGoogle Scholar
  11. 11.
    Takahara J., Yunoki S., Hosogi H., Yakushiji W., Kageyama J., Ofuji T. Concomitant increases in serum growth hormone and hypothalamic somatostatin in rats after injection of γ-aminobutyric acid, aminooxyacetic acid, or γ-hydroxy-butyric acid. Endocrinology 106: 343, 1980.PubMedCrossRefGoogle Scholar
  12. 12.
    Fioretti P., Melis G.B., Paoletti A.M., Parodo G., Caminiti F., Corsini G.U., Martini L. γ-amino-β-hydroxybutyric acid stimulates prolactin and growth hormone release in normal women. J. Clin. Endocrinol. Metab. 47: 1336, 1978.PubMedCrossRefGoogle Scholar
  13. 13.
    Mac Leod R.M., Robyn C. Mechanism of increased prolactin secretion by sulpiride. J. Endocrinol. 72: 273, 1977.CrossRefGoogle Scholar
  14. 14.
    Brazeau P., Vale W., Burgus R., Ling N., Butcher M., Rivier J., Guillemin R. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science 179: 77, 1973.PubMedCrossRefGoogle Scholar
  15. 15.
    Serially A.V., Dupont A., Arimura A., Redding T.W., Nishi N. Linthicum G.L., Schlesinger D.H. Isolation and structure of somatostatin from porcine hypothalami. Biochemistry 15: 509, 1976.CrossRefGoogle Scholar
  16. 16.
    Vale W., Rivier C., Brown M. Regulatory peptides of the hypothalamus. Annu. Rev. Physiol. 39: 473, 1977.PubMedCrossRefGoogle Scholar
  17. 17.
    Chihara K., Arimura A., Chihara M., Schally A.V. Studies on the mechanism of growth hormone and thyrotropin rsponses to somatostatin antiserum in anesthetized rats. Endocrinology 103: 1916, 1978.PubMedCrossRefGoogle Scholar
  18. 18.
    Chihara K., Arimura A., Schally A.V. Effect of intraventricular injection of dopamine, norepinephrine, acetylcholine, and 5-hydroxytryptamine on immunoreactive somatostatin release into rat hypophyseal portal blood. Endocrinology 104: 1656, 1979.PubMedCrossRefGoogle Scholar
  19. 19.
    Collu R., Du Ruisseau P., Taché Y. Role of putative neurotransmitters in prolactin, GH and LH response to acute immobilization stress in male rats. Neuroendocrinology 28: 178, 1979.PubMedCrossRefGoogle Scholar
  20. 20.
    Locatelli V., Panerai A.E., Cocchi D., Gil-Ad I., Mantegazza P., Secchi C., Müller E.E. Drug-induced changes of brain serotoninergic tone and insulin-induced growth hormone release in the dog. Neuroendocrinology 25: 84, 1978.PubMedCrossRefGoogle Scholar
  21. 21.
    Martin J.B., Durand D., Gurd W., Faille G., Audet J., Brazeau P. Neuropharmacological regulation of episodic growth hormone and prolactin secretion in the rat. Endocrinology 102: 106, 1978.PubMedCrossRefGoogle Scholar
  22. 22.
    Woolf P.D., Lantigua R., Lee L.A. Dopamine inhibition of stimulated growth hormone secretion: evidence for dopaminergic modulation of insulin and L-dopa-induced growth hormone secretion in man. J. Clin. Endocrinol. Metab. 49: 326, 1979.PubMedCrossRefGoogle Scholar
  23. 23.
    Giorguief F.M.F., Kernel M.L., Glowinski J. Besson M.J. Stimulation of dopamine release by GABA in rat striatal slices. Brain Res. 139: 115, 1978.CrossRefGoogle Scholar
  24. 24.
    Yessaian N.H., Armenian A.R., Buniatian H.Ch. Effect of gamma-amino-butyric acid on brain serotonin and catecholamines. J. Neurochem. 16: 1425, 1969.PubMedCrossRefGoogle Scholar
  25. 25.
    Nistri A., Costanti A. Some observations on the mechanism of action of baclofen (Beta-chforo phenyl γ-amino-butyric acid). Experientia 31: 64, 1975.PubMedCrossRefGoogle Scholar
  26. 26.
    Pycock C., Horton R. Regional changes in the concentrations of cerebral monoamines and their metabolites after ethanolamine-0-sulphate induced elevation of brain γ-aminobutyric acid concentrations. Biochem. Pharmacol. 27: 1827, 1978.PubMedCrossRefGoogle Scholar
  27. 27.
    Weiner R.I., Ganong W.F. Role of brain monoamines and histamine in regulation of anterior pituitary secretion. Physiol. Rev. 58: 905, 1978.PubMedGoogle Scholar
  28. 28.
    Melis G.B., Paoletti A.M., Mais V., Fioretti P. Interference of dopamine infusion on γ-amino butyric acid (GABA)-stimulated prolactin increase. J. Endocrinol. Invest. 3: 445, 1980.PubMedCrossRefGoogle Scholar
  29. 29.
    Vijayan E., McCann S.M. Blockade of dopamine (DA) receptors with pimozide and pituitary hormone release in response to intraventricular injection of γ-aminobutyrid acid (GABA) in conscious ovariectomized rats. Brain Res. 162: 69, 1979.PubMedCrossRefGoogle Scholar
  30. 30.
    L’Hermite M., Denayer P., Golstein J., Virasoro E., Vanhaelst L. Copinschi G. Robyn C. Acute endocrine profile of sulpiride in the human. Clin. Endocrinol. (Oxf.) 9: 195, 1978.CrossRefGoogle Scholar
  31. 31.
    Cavagnini F., Benetti G., Invitti C., Ramella G., Pinto M., Lazza M., Dubini A., Marelli A., Müller E.E. Effect of γ-aminobutyric acid on growth hormone and prolactin secretion in man: influence of pimozide and domperidone. J. Clin. Endocrinol. Metab. 51: 789, 1980.PubMedCrossRefGoogle Scholar
  32. 32.
    Andén N.E., Butcher S.G., Corrodi H., Fuxe K., Ungerstedt U. Receptor activity and turnover of dopamine and noradrenaline after neuroleptics. Eur. J. Pharmacol. 11: 303, 1970.PubMedCrossRefGoogle Scholar
  33. 33.
    Delitala G. Stimulating action of sulpiride and pimozide on prolactin release. Effect on bromocriptine, L-dopa and metergoline administration. Acta Endocrinol. (Kbh.) 86: 251, 1977.Google Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1982

Authors and Affiliations

  • Gian B. Melis
    • 1
  • A. M. Paoletti
    • 1
  • V. Mais
    • 1
  • N. M. Mastrapasqua
    • 1
  • F. Strigini
    • 1
  • F. Fruzzetti
    • 1
  • G. Guarnieri
    • 1
  • M. Gambacciani
    • 1
  • P. Fioretti
    • 1
  1. 1.Clinica Ostetrica e GinecologicaUniversità di PisaPisaItaly

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