Advertisement

Journal of Endocrinological Investigation

, Volume 11, Issue 1, pp 37–42 | Cite as

Plasma β-endorphin, free fatty acids and blood lipid changes in type 2 (non-insulin dependent) diabetic patients

  • S. B. Solerte
  • M. Fioravanti
  • F. Petraglia
  • F. Facchinetti
  • A. L. Patti
  • N. Schifino
  • A. R. Genazzani
  • E. Ferrari
Article

Abstract

A lipolytic activity for β-endorphin (βEP) has been recently suggested both in vitro and in vivo. In our study we evaluated the relationship between βEP and blood lipid pattern in Type 2 (non-insulin dependent) diabetic patients. Plasma βEP, together with plasma β-lipotropin (βLPH), ACTH, cortisol and plasma insulin (IRI), was measured by RIA after silicic acid plasma extraction and Sephedex G-75 column chromatography. Although reduced βEP (7.12 ± 3.8 fmol/ml) and increased βLPH (9.3 ± 3.7 fmol/ml) levels were found in diabetic patients, compared to controls (8.53 ± 3.3 fmol/ml, p< 0.05 and 8.34 ± 2.6 fmol/ml, p< 0.05, respectively), higher plasma βEP concentrations were demonstrated in hyperlipidemic diabetic patients (10.3 ± 3.9 fmol/ml) than in patients with normal blood lipid pattern (4.85 ± 1.45 fmol/ml, p< 0.001 ). Several positive correlations between βEP, plasma free fatty acids (r = 0.75, p< 0.001 ), triglycerides (r = 0.84, p< 0.001 ) and VLDL (r = 0.80, p< 0.001 ) were found in our patients independently of overweight, hypoglycemic treatment, plasma IRI levels and of the degree of metabolic control. A higher prevalence of micro- and macrovascular complications was demonstrated in hyperlipidemic than in normolipidemic patients. Blood lipid disorders mighttherefore be associated with increased plasma βEP levels in Type 2 diabetes.

En]Keywords

Non-insulin dependent diabetes β-endorphin blood lipids free fatty acids apolipoprotein B angiopathy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Morley J.E. The endocrinology of the opiates and opioid peptides. Metabolism 30: 195, 1981.PubMedCrossRefGoogle Scholar
  2. 2.
    Grube D., Voight K.H., Weber E. Pancreatic glucagon cells contain endorphin-like immunoreactivity. Histochemistry 59: 75, 1978.PubMedCrossRefGoogle Scholar
  3. 3.
    Watkins W.B., Bruni J.F., Yen S.S.C. β-Endorphin and somatostatin in the pancreatic d-cells: colocalization by immunocytochemistry. J. Histochem. Cytochem. 28: 1170, 1980.PubMedCrossRefGoogle Scholar
  4. 4.
    Bruni J.F., Watkins W.B., Yen S.S.C. β-Endorphin in the human pancreas. J. Clin. Endocrinol. Metab. 49: 649, 1979.PubMedCrossRefGoogle Scholar
  5. 5.
    Tung A.K., Cockburn E. β-Endorphin-like immunoreactivity in extracts of the fetal bovine pancreas. Column chromatographic characterization of a high-molecular-weight immunoreactive species. Diabetes 33: 235, 1984.PubMedCrossRefGoogle Scholar
  6. 6.
    Ipp E., Dobbs R., Unger R.H. Morphine and β-endorphin influence the secretion of the endocrine pancreas. Nature 276: 190, 1978.PubMedCrossRefGoogle Scholar
  7. 7.
    Reid R.L., Yen S.S.C. β-Endorphin stimulates the secretion of insulin and glucagon in humans. J. Clin. Endocrinol. Metab. 52: 592, 1981.PubMedCrossRefGoogle Scholar
  8. 8.
    Reid R.L., Sandler J.A., Yen S.S.C. β-Endorphin stimulates the secretion of insulin and glucagon in diabetes mellitus. Metabolism 33: 197, 1984.PubMedCrossRefGoogle Scholar
  9. 9.
    Awoke S., Voyles N.R., Bhathena S.J., Tanenberg R.J., Recant L. Alterations of plasma opioid activity in human diabetics. Life Sci. 34: 1999, 1984.PubMedCrossRefGoogle Scholar
  10. 10.
    Vermes I., Steinmetz E., Schoorl J., Van der Veen E.A., Tilders F.J.H. Increased plasma levels of immunoreactive β-endorphin and corticotropin in non-insulin dependent diabetes. Lancet 2: 725, 1985.PubMedCrossRefGoogle Scholar
  11. 11.
    Solerte S.B., Petraglia F., Facchinetti F., Genazzani A.R., Ferrari E. Dissociation between β-lipotropin and ACTH secretion in Type I and Type II diabetes mellitus. J. Endocrinol. Invest. 8: (Suppl. 3) 197, 1985.Google Scholar
  12. 12.
    Solerte S.B., Fioravanti M., Petraglia F., Facchinetti F., Aprile C., Genazzani A.R., Ferrari E. Circulating opioids and plasma renin activity in insulin-dependent diabetics with renal haemodynamic alterations. Nephron 46: 194, 1987.PubMedCrossRefGoogle Scholar
  13. 13.
    Richter W.O., Schwandt P. In vitro lipolysis of proopiocortin peptides. Life Sci. 33: (Suppl. 1) 747, 1983.PubMedCrossRefGoogle Scholar
  14. 14.
    Richter W.O., Kerscher P., Schwandt P. β-Endorphin stimulates in vivo lipolysis in the rabbit. Life Sci. 33: (Suppl. 1) 743, 1983.PubMedCrossRefGoogle Scholar
  15. 15.
    Genazzani A.R., Nappi G., Facchinetti F., Mazzella G.L., Parrini D., Sinforiani E., Petraglia F., Savoldi F. Central deficiency of β-endorphin in alcohol addicts. J. Clin. Endocrinol. Metab. 55: 583, 1982.PubMedCrossRefGoogle Scholar
  16. 16.
    Genazzani A.R., Facchinetti F., Pintor C., Puggioni R., Parrini D., Petraglia F., Bagnoli F., Corda R. Proopiomelanocortin-related peptide plasma levels throughout prepuberty and puberty. J. Clin. Endocrinol. Matab. 57: 56, 1983.CrossRefGoogle Scholar
  17. 17.
    Genazzani A.R Facchinetti F., Petraglia F., Pintor C., Corda R. Hyperendorphinemia in obese children and adolescents. J. Clin. Endocrinol. Metab. 62: 36, 1986.PubMedCrossRefGoogle Scholar
  18. 18.
    Soloni F.G., Sardina L.C. Colorimetric microdetermination of free fatty acids. Clin. Chem. 19: 419, 1973.PubMedGoogle Scholar
  19. 19.
    Allein C.C., Poon L.S., Chan C.S.G., Richmond W., Fu P.C. Enzymatic determination of total serum cholesterol. Clin. Chem. 20: 470, 1974.Google Scholar
  20. 20.
    Bucolo G., David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin. Chem. 19: 476, 1973.PubMedGoogle Scholar
  21. 21.
    Friedewald W.T., Levy R.I., Fredrickson D.S. Estimation of the concentration of low density cholesterol in plasma without use of the preparative ultracentrifuge. Clin. Chem. 18: 499, 1972.PubMedGoogle Scholar
  22. 22.
    Wamick G.R., Albers J.J. Comprehensive evaluation of the heparin manganese precipitation procedure for the estimation of high density lipoprotein cholesterol. J. Lipid Res. 19: 65, 1978.Google Scholar
  23. 23.
    Noble R.P., Hatch F.F., Mazzimas J.A., Lindgren F.T., Jensen L.C., Adamson G.L. Comparison of lipoprotein analysis by agarose gel and paper electrophoresis with analytical ultracentrifugation. Lipids 4: 55, 1969.PubMedCrossRefGoogle Scholar
  24. 24.
    Laurell C. Quantitative estimation of protein by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 15: 45, 1966.PubMedCrossRefGoogle Scholar
  25. 25.
    Giugliano D., Ceriello A., Quatraro A., D’Onofrio F. Endogenous opiates, heroin addiction, and non-insulin-dependent diabetes. Lancet 2: 769, 1985.PubMedCrossRefGoogle Scholar
  26. 26.
    Giugliano D., Ceriello A., Salvatore T., Paolisso G., D’Onofrio F., Lefèbvre P. β-endorphin infusion restores acute insulin responses to glucose in Type-2 diabetes mellitus. J. Clin. Endocrinol. Metab. 64: 944, 1987.PubMedCrossRefGoogle Scholar
  27. 27.
    Giugliano D., Salvatore T., Cozzolino D., Ceriello A., Torella R., D’Onofrio F. Hyperglycemia and obesity as determinants of glucose, insulin and glucagon responses to β-endorphin in human diabetes mellitus. J. Clin. Endocrinol. Metab. 64: 1122, 1987.PubMedCrossRefGoogle Scholar
  28. 28.
    Givens J.R., Wiedeman E., Andersen R.N., Kitabchi A.E. β-endorphin and β-lipotropin plasma levels in hirsute women: correlation with body weight. J. Clin. Endocrinol. Metab. 50: 975, 1980.PubMedCrossRefGoogle Scholar
  29. 29.
    Morley J.E., Levine A.S. The central control of appetite. Lancet 1: 398, 1983.PubMedCrossRefGoogle Scholar
  30. 30.
    Vettor R., De Carlo E., Manno M., Martini C., Maran A., Scandellari C., Federspil G. Peripheral effects of opioids: studies on in vitro lipolysis. J. Endocrinol. Invest. 10: (Suppl. 1) 68, 1987.Google Scholar
  31. 31.
    Steiner G. Diabetes and atherosclerosis. Diabetes 30 (Suppl. 2): 1, 1981.PubMedCrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1988

Authors and Affiliations

  • S. B. Solerte
    • 1
  • M. Fioravanti
    • 1
  • F. Petraglia
    • 2
  • F. Facchinetti
    • 2
  • A. L. Patti
    • 1
  • N. Schifino
    • 1
  • A. R. Genazzani
    • 2
  • E. Ferrari
    • 1
  1. 1.Dipartimento di Medicina InternaClinica Medica II, University of PaviaPaviaItaly
  2. 2.Clinica Ostetrico-GinecologicaUniversity of ModenaModenaItaly

Personalised recommendations