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Effect of amiodarone on L-triiodothyronine stimulation of [3H] thymidine incorporation into GH3 cells

  • I. D. Goldfine
  • B. Maddux
  • K. A. Woeber
Article

Abstract

The antiarrhythmic agent amiodarone was found to inhibit the stimulatory effects of L-triiodothyronine on [3H ] thymidine incorporation into GH3 rat pituitary tumor cells. This inhibitory effect of amiodarone was detected at concentrations as low as 0.5 μM; at 2 μM greater than 50% of the stimulatory effect of L-triiodothyronine was inhibited. The effect of amiodarone was present at all concentrations of L-triiodothyronine tested (50 pM to 10 nM), suggesting that amiodarone acted as a non-competitive antagonist. These studies raise the possibility, therefore, that the effect of amiodarone on thyroid hormone metabolism may be mediated in part by an inhibition of thyroid hormone action at the cellular level.

Key-words

L-triiodothyronine amiodarone GH3 cells 

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References

  1. 1.
    Vastesaeger M., Gillot P., Rasson G. Etude clinique d’une nouvelle médication antiangoreuse. Acta Cardiol. (Brux.) 22: 483, 1967.Google Scholar
  2. 2.
    Rosenbaum, M.B., Chiale, P.A., Halpern M.S., Nau G.J., Przybylski J., Levi R.J., Lazzari J.O., Elizari M.V. Clinical efficacy of amiodarone as an antiarrhythmic agent. Am. J. Cardiol. 38: 934, 1976.PubMedCrossRefGoogle Scholar
  3. 3.
    Cote P., Bourassa M.G., Delaye J., Janin A., Froment R., David P. Effects of amiodarone on cardiac and coronary hemodynamics and on myocardial metabolism in patients with coronary artery disease. Circulation 59: 1165, 1979.PubMedCrossRefGoogle Scholar
  4. 4.
    Burger A., Dinichert D., Nicod P., Jenny M., Lemarchand-Béraud T., Vallotton M.B. Effect of amiodarone on serum triiodothyronine, reverse triiodothyronine, thyroxin, and thyrotropin. A drug influencing peripheral metabolism of thyroid hormones. J.Clin. Invest. 58: 255, 1976.PubMedCentralPubMedCrossRefGoogle Scholar
  5. 5.
    Samuels HH., Tsai J.S., Cintron R. Thyroid hormone action: A cell-culture system responsive to physiological concentrations of thyroid hormones. Science 181: 1253, 1973.PubMedCrossRefGoogle Scholar
  6. 6.
    Lowry O.H., Rosebrough N.J., Farr A.L, Randall R. Protein measurement with the Folin Phenol reagent. J. Biol. Chem. 193: 265, 1951.PubMedGoogle Scholar
  7. 7.
    Bondar R.J.L., Mead D. Evaluation of glu.cose-6-phosphate dehydrogenase from leuconostoc mesenteroides in the hexokinase method for determining glucose in serum. Clin. Chem. 20: 586, 1974.PubMedGoogle Scholar
  8. 8.
    Polster P., Broekhuysen J. The adrenergic antagonism of amiodarone. Biochem. Pharmacol. 25: 131, 1976.PubMedCrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1982

Authors and Affiliations

  • I. D. Goldfine
    • 1
    • 2
    • 3
  • B. Maddux
    • 1
  • K. A. Woeber
    • 1
    • 2
  1. 1.Cell Biology Laboratory and Department of MedicineMount Zion Hospital and Medical CenterSan FranciscoUSA
  2. 2.Department of MedicineUniversity of CaliforniaSan FranciscoUSA
  3. 3.Department of PhysiologyUniversity of CaliforniaSan FranciscoUSA

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