Journal of Endocrinological Investigation

, Volume 19, Issue 8, pp 530–534 | Cite as

Effect of dexamethasone on insulin receptor mRNA levels, RNA stability and isotype RNA pattern in U-937 human promonocytic cells

  • M. A. Leal
  • P. Aller
  • C. Calle
Article
First Online:
Accepted:

Abstract

The administration of 5×106 mol/l dexamethasone transiently increased insulin receptor (IR) mRNA levels in U-937 human promonocytic cells, which reached the maximum level at 15 h of treatment. Dexamethasone treatment did not affect the IR mRNA half-life (approximately 4 h), suggesting that the increase is regulated at the transcriptional level. The stimulatory action of dexamethasone was not prevented by the simultaneous presence of the protein synthesis inhibitor cycloheximide, indicating that the induction of IR gene transcription occurs as a direct response to the action of the synthetic glucocorticoid. Finally, the A isoform (lacking exon 11) was found to be the only IR isoform present in both untreated and dexamethasone treated-U-937 cells.

Keywords

Insulin receptor gene expression dexamethasone U-937 cells insulin receptor isotypes 
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Copyright information

© Italian Society of Endocrinology (SIE) 1996

Authors and Affiliations

  • M. A. Leal
    • 1
  • P. Aller
    • 2
  • C. Calle
    • 1
  1. 1.Departamento de Bioquímica, Facultad de MedicinaUniversidad ComplutenseMadridSpain
  2. 2.Centro de Investigaciónes BiológicasC.S.I.C.MadridSpain

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