Nuclear DNA content of medullary thyroid carcinoma in a large family with the MEN-2A syndrome
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Abstract
Flow cytometry of medullary thyroid carcinoma (MCT) was performed in a large family with the MEN-2A syndrome. Of 15 family members with MCT five patients (10–27 yr) were without lymph node metastases. Six patients had a normal pentagastrin test after operation. All patients are alive and free of symptoms of MCT 6–9 yr after total thyroidectomy and an ablative dose of 131-I. In 12 of the 15 patients with MCT flowcytometry of paraffin-embedded tissue could be performed. The majority of all tumors (n=9) were classified as peridiploid. Metastatic tumor, 6 years after thyroidectomy, in one of the patients was diploid. Only two MCT were clearly aneuploid. In one patient the tumor was tetraploid. We conclude that the majority of the MCT patients in this family with the MEN-2A syndrome have no or limited ploidy aberrations in their tumors, which correlates well with the favourable prognosis of familial MCT.
Key-words
Medullary thyroid carcinoma MEN-2A syndrome flow cytometry prognostic factorsPreview
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References
- 1.Vasen H.F.A., Nieuwenhuijzen Kruseman A.C., Berkel H., Beukers E.K.M., Delprat C.C., van Doorn R.L.G., Geerdink R.A., Haak H.R., Hackeng W.H.L., Koppeschaar H.P.F., Kreuning E.P., Lamberts S.J., Lekkerkerker F.J.F., Michels R.P.J., Moers A.M.J., Pieters G.F.F.M., Wiersinga W.M., Ups C.J.M. Multiple endocrine neoplasia syndrome type 2: the value of screening and central registration. Am. J. Med. 83: 847, 1987.PubMedCrossRefGoogle Scholar
- 2.Friedlander M.L., Hedley D.W., Taylor I.W. Clinical and biological significance of aneuploidy in human tumors. J. Clin. Pathol. 37: 961, 1989.CrossRefGoogle Scholar
- 3.Hamming J.F., Schelfhout L.J.D.M., Cornelisse C.J., van de Velde C.J.H., Goslings B.M., Hermans J., Fleuren G.J. Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer. World J. Surg 12: 503, 1988.PubMedCrossRefGoogle Scholar
- 4.Bergholm N., Adami H.O., Auer G. Histopathologic characteristics and nuclear DNA content as prognostic factors in medullary thyroid carcinoma. Cancer 64: 135, 1989.PubMedCrossRefGoogle Scholar
- 5.Haak H.R., Nieuwenhuijzen Kruseman A.C. Characteristics of a family with the MEN-2A syndrome. Henry Ford Hosp. Med. J. 35: 104, 1987.PubMedGoogle Scholar
- 6.Hedley D.W., Friedlander M.L., Taylor I.W., Rugg C.A., Musgrove E.A. Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. J. Histochem. Cytochem. 31: 1333, 1983.PubMedCrossRefGoogle Scholar
- 7.Gautvik K.M., Talle K., Hager B., Jorgensen O.G., Aas M. Early liver metastases in patients with medullary carcinoma of the thyroid gland. Cancer 63: 175, 1989.PubMedCrossRefGoogle Scholar
- 8.Saad M.F., Ordonez N.G., Rashid R.K., Guido J.J., Hill C.S., Hickey R.C., Samaan N.A. Medullary carcinoma of the thyroid: A study of the clinical features and prognostic factors in 161 patients. Medicine (Baltimore) 63: 319, 1984.CrossRefGoogle Scholar
- 9.Samaan N.A., Schultz P.N., Hickey R.C. Medullary thyroid carcinoma: Prognosis of familial versus nonfamilial disease and the role of radiotherapy. Horm. Metab. Res. 21: 21, 1989.Google Scholar
- 10.Hedley D.W., Friedlander M.L., Taylor I.W. Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. Cytometry 6: 327, 1985.PubMedCrossRefGoogle Scholar
- 11.Rodenburg C.J., Cornelisse C.J., Heintz P.A.M., Hermans J., Fleuren G.J. Tumor ploidy as a major prognostic factor in advanced ovarian cancer. Cancer 59: 317, 1987.PubMedCrossRefGoogle Scholar
- 12.Hay I.D., Ryan J.J., Grant C.S., Bergstrahl E.J., van Heerden J.A., Goellner J.R. Flow cytometric nuclear DNA ploidy determination: A significant predictor of outcome in sporadic and familial medullary thyroid carcinoma. EORTC Thryroid Study Group, Malmö, Sweden, 1989 p. 16 (Abstract).Google Scholar