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Journal of Endocrinological Investigation

, Volume 32, Issue 11, pp 873–876 | Cite as

Pluripotent stem cells isolated from human amniotic fluid and differentiation into pancreatic β-cells

  • L. Trovato
  • R. De Fazio
  • M. Annunziata
  • S. Sdei
  • E. Favaro
  • R. Ponti
  • L. Marozio
  • E. Ghigo
  • C. Benedetto
  • R. Granata
Rapid Communication

Abstract

Human amniotic fluid (HAF) contains multipotent stem cells [amniotic fluid-derived stem cells (AFSC)] which can differentiate into a variety of different cell types. Recently, we demonstrated that obestatin, a peptide encoded by the ghrelin gene, exerts anti-apoptotic effects in pancreatic β-cells and human islets and increases the expression of genes involved in β-cells differentiation. We investigated whether: 1) AFSC would differentiate into pancreatic β-cells and 2) obestatin would increase β-cells differentiation from AFSC. Fluorescence-activated cell sorting analysis and immunocytochemical staining showed the presence of mesenchymal and endothelial markers in AFSC. Real-time PCR evidenced the expression of Octamer binding transcription factor 4 (OCT-4), a marker of pluripotency, during the early differentiation phase. However, the β-cells differentiation marker duodenal homeobox factor-1 (PDX-1) could not be detected. Obestatin increased OCT-4 expression but had no effect on β-cells differentiation. These results suggest that, at least under the experimental conditions used in this study, AFSC do not differentiate into β-cells and obestatin has no additional effect.

Keywords

Amniotic fluid-derived stem cells differentiation pancreatic β-cells obestatin 

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Copyright information

© Italian Society of Endocrinology (SIE) 2009

Authors and Affiliations

  • L. Trovato
    • 1
    • 2
  • R. De Fazio
    • 3
  • M. Annunziata
    • 1
    • 2
  • S. Sdei
    • 3
  • E. Favaro
    • 2
  • R. Ponti
    • 4
  • L. Marozio
    • 3
  • E. Ghigo
    • 1
    • 2
  • C. Benedetto
    • 3
  • R. Granata
    • 1
    • 2
  1. 1.Division of Endocrinology and MetabolismUniversity of TurinTurinItaly
  2. 2.Department of Internal MedicineUniversity of TurinTurinItaly
  3. 3.Department of Gynecology and ObstetricsUniversity of TurinTurinItaly
  4. 4.Department of Biomedical Science and Human OncologyUniversity of TurinTurinItaly

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