Journal of Endocrinological Investigation

, Volume 32, Issue 8, pp 671–676 | Cite as

Establishment of reference serum insulin-like growth factor-I and insulin-like growth factor binding protein-3 levels of Tunisian children during childhood and adolescence

  • S. Sfar
  • M. Zaouali Ajina
  • S. Ben Mdalla
  • A. Baklouti
  • H. Knani
  • Z. Tabka
  • A. Zbidi
Original Articles
First Online:
Accepted:

Abstract

Background: The aim of the present study is to establish age- and sex-related reference ranges of serum IGF-I and IGF binding protein-3 (IGFBP-3) levels in a pattern of Tunisian children. Subjects and methods: Two hundred healthy Tunisian children (103 boys and 97 girls), aged between 6 and 16 yr, were considered in the study. Results: Mean serum levels of IGF-I and IGFBP-3 are observed to be higher in girls compared to boys of the same age interval. However, these differences were statistically significant only in pubertal ages (11–14 yr) for IGF-I and in pre-pubertal ages (6–10 yr) for IGFBP-3 (p<0.05). Steeper variations in IGF-I concentrations were obtained earlier in girls than in boys (11–12 vs 12–13 yr, Tanner stage 3–4). Peak of IGF-I levels are observed at almost the same age interval (12–14 yr). IGFBP-3 levels significantly increased at steeper variations of IGF-I for both sexes followed by steady values. Conclusions: Variations of IGF-I and IGFBP-3 with the considered parameters (sex, age, and puberty stage), which concord with previous studies on various populations, emphasize the importance of locally established reference levels to construct a SD score prediction model. Establishment of reference serum IGF-I and IGFBP-3 ranges during childhood and adolescence in Tunisian subjects can help to enhance the diagnostic efficiency of IGF-I and IGFBP-3 in evaluating growth disorders in our population.

Keywords

Healthy children insulin-like growth factor-I insulin-like growth factor binding protein-3 puberty reference ranges 
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References

  1. 1.
    Gohlke BC, Frazer FL, Stanhope R. Growth hormone secretion and long-term growth data in children with psychosocial short stature treated by different changes in environment. J Pediatr Endocrinol Metab 2004, 17: 637–43.PubMedCrossRefGoogle Scholar
  2. 2.
    Löfqvist C, Andersson E, Gelander L, Rosberg S, Blum WF, Albertsson Wikland K. Reference values for IGF-I throughout childhood and adolescence: a model that accounts simultaneously for the effect of gender, age, and puberty. J Clin Endocrinol Metab 2001, 86: 5870–6.PubMedCrossRefGoogle Scholar
  3. 3.
    Chevenne D, Porquet D. Hormone de croissance (GH) et insulin-like growth factor I (IGF-1) dans les états nutritionnels. Ann Biol Clin (Paris) 1995, 53: 527–38.Google Scholar
  4. 4.
    Sara V, Hall K. Insulin-like growth factors and their binding proteins. Physiol Rev 1990, 70: 591–614.PubMedGoogle Scholar
  5. 5.
    Blum WF, Albertsson-Wikland K, Rosberg S, Ranke MB. Serum levels of insulin-like growth factor I (IGF-I) and IGF binding protein 3 reflect spontaneous growth hormone secretion. J Clin Endocrinol Metab 1993, 76: 1610–6.PubMedGoogle Scholar
  6. 6.
    Juul A, Skakkebaek NE. Prediction of the outcome of growth hormone provocative testing in short children by measurement of serum levels of insulin-like growth factor I and insulin-like growth factor binding protein 3. J Pediatr 1997, 130: 197–204.PubMedCrossRefGoogle Scholar
  7. 7.
    Jaruratanasirikul S, Leethanaporn K, Sriplung H, et al. The usefulness of serum insulin-like growth factor-1 (IGF1) and insulin-like growth factor binding protein-3 (IGFBP3) for evaluation of children with short stature. J Med Assoc Thai 2000, 83: 619–26.PubMedGoogle Scholar
  8. 8.
    Blum WF, Ranke MB. Use of insulin-like growth factor-binding protein 3 for the evaluation of growth disorders. Horm Res 1990, 33: 31–7.PubMedCrossRefGoogle Scholar
  9. 9.
    Stoffel-Wagner B, Springer W, Bidlingmaier F, Klingmüller D. A comparison of different methods for diagnosing acromegaly. Clin Endocrinol (Oxf) 1997, 46: 531–7.CrossRefGoogle Scholar
  10. 10.
    Argente J, Barrios V, Pozo J, et al. Normative data for insulin-like growth factors (IGFs), IGF-binding proteins, and growth hormone-binding protein in a healthy Spanish pediatric population: age- and sex-related changes. J Clin Endocrinol Metab 1993, 77: 1522–8.PubMedGoogle Scholar
  11. 11.
    Juul A, Dalgaard P, Blum WF, et al. Serum levels of Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) in healthy infants, children, and adolescents: the relation to IGF-I, IGF-II, IGFBP-1, IGFBP-2, age, sex, body mass index, and pubertal maturation. J Clin Endocrinol Metab 1995, 80: 2534–42.PubMedGoogle Scholar
  12. 12.
    Löfqvist C, Anderson E, Gelander L, et al. Reference values for insulin-like growth factor-binding protein-3 (IGFBP-3) and the ratio of insulin-like growth factor-I to IGFBP-3 throughout childhood and adolescence. J Clin Endocrinol Metab 2005, 90: 1420–7.PubMedCrossRefGoogle Scholar
  13. 13.
    Bereket A, Turan S, Omar A, et al. Serum IGF-I and IGFBP-3 levels of Turkish children during childhood and adolescence: establishment of reference ranges with emphasis on puberty. Horm Res 2006, 65: 96–105.PubMedCrossRefGoogle Scholar
  14. 14.
    Elmlinger MW, Kühnel W, Weber MM, Ranke MB. Reference ranges for two automated chemiluminescent assays for serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3). Clin Chem Lab Med 2004, 42: 654–64.PubMedCrossRefGoogle Scholar
  15. 15.
    Sempé M, Pédron G. Auxologie, méthode et séquences. Paris: Laboratoire Theraplix, 1979.Google Scholar
  16. 16.
    Miles LE, Lipschitz DA, Bieber CP, Cook JD. Measurement of serum ferritin by a 2-site radioimmunoradiometric assay. Anal Biochem 1974, 61: 209–24.PubMedCrossRefGoogle Scholar
  17. 17.
    Daughaday WH, Rotwein P. Insulin-like growth factors I and II. Peptide, messenger ribonucleic acid and gene structures, serum and tissue concentrations. Endocr Rev 1989, 10: 68–91.PubMedCrossRefGoogle Scholar
  18. 18.
    Juul A, Bang P, Hertel NT, et al. Serum insulin-like growth factor-1 in 1030 healthy children, adolescents and adults; relation to age, sex, stage of puberty, testicular size and body mass index. J Clin Endocrinol Metab 1994, 78: 744–52.PubMedGoogle Scholar
  19. 19.
    Kawai N, Kanzaki S, Takano-Watou S, et al. Serum-free insulin-like growth factor I (IGF-I), total IGF-I, and IGF-binding protein-3 concentrations in normal children and children with growth hormone deficiency. J Clin Endocrinol Metab 1999, 84: 82–9.PubMedGoogle Scholar
  20. 20.
    Brabant G, von zur Mühlen A, Wüster C, et al. Serum insulin-like growth factor I reference values for an automated chemiluminescence immunoassay system: result from a multicenter study. Horm Res 2003, 60: 53–60.PubMedCrossRefGoogle Scholar
  21. 21.
    Kong APS, Wong GWK, Choi KC, et al. Reference values for serum levels of insulin-like growth factor (IGF-1) and IGF-binding protein 3 (IGFBP-3) and their ratio in Chinese adolescents. J Clin Biochem 2007, 40: 1093–9.CrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 2009

Authors and Affiliations

  • S. Sfar
    • 1
  • M. Zaouali Ajina
    • 1
  • S. Ben Mdalla
    • 1
  • A. Baklouti
    • 1
  • H. Knani
    • 2
  • Z. Tabka
    • 1
  • A. Zbidi
    • 1
  1. 1.Laboratory of Physiology and Functional Explorations, Faculty of Medicine Ibn El-JazzarSousseTunisia
  2. 2.Primary Health Care ServiceSousseTunisia

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