Journal of Endocrinological Investigation

, Volume 29, Issue 2, pp 147–153 | Cite as

Expression of potassium channel isoforms mRNA in normal human adrenals and aldosterone-secreting adenomas

  • R. Sarzani
  • F. Pietrucci
  • M. Francioni
  • F. Salvi
  • C. Letizia
  • E. D’Erasmo
  • P. Dessì Fulgheri
  • A. Rappelli
Original Articles
First Online:
Accepted:

Abstract

Increased aldosterone secretion has been found in a mouse lacking the KCNE1 gene which codes for a regulatory protein of the KCNQ1 gene product, forming the channel for the outward rectifying delayed K+ current. Abnormalities in proteins regulating the K+ fluxes across membranes may be responsible for aldosterone-secreting adenomas (aldosteronomas) also because K+ channels are involved in cell growth. Normal and adenomatous adrenal samples and NCI-H295 cell line were used to: a) evaluate KCNE1 and KCNQ1 gene expression, b) sequence the full length cD-NAs of KCNE1 and both KCNQ1 isoforms. These differently spliced KCNE1 and KCNQ1 mRNAs were expressed in adrenal tissue. In contrast, KC-NQ1 isoform 2 mRNA was not expressed in kidney control tissues and NCI-H295 cell line. NCI-H295 cell line also had a significantly lower expression of KCNQ1 isoform 1 mRNA than normal adrenals and aldosteronomas. We did not find any somatic mutations in the coding sequences of both genes. This different expression pattern of KCNQ1 isoforms in NCI-H295 cell line with the lack of the mRNA for the dominant-negative KCNQ1 isoform 2 supports the involvement of voltage-gated K+ channel in cell proliferation.

En]Keywords

Aldosterone secreting adenomas KCNQ1 KCNE1 
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Copyright information

© Italian Society of Endocrinology (SIE) 2006

Authors and Affiliations

  • R. Sarzani
    • 1
  • F. Pietrucci
    • 1
  • M. Francioni
    • 1
  • F. Salvi
    • 1
  • C. Letizia
    • 2
  • E. D’Erasmo
    • 2
  • P. Dessì Fulgheri
    • 1
  • A. Rappelli
    • 1
  1. 1.Department of Internal MedicinePolytechnical University of Marche, Ospedali Riuniti “Umberto I — Lancisi — Salesi”AnconaItaly
  2. 2.Department of Clinical SciencesUniversity of Rome “La Sapienza”RomeItaly

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