Biodegradation of polycyclic aromatic hydrocarbon by a halotolerant bacterial consortium isolated from marine environment
- 326 Downloads
The biodegradability of polycyclic aromatic hydrocarbons such as naphthalene, fluorene, anthracene and phenanthrene by a halotolerant bacterial consortium isolated from marine environment was investigated. The polycyclic aromatic hydrocarbons degrading bacterial consortium was enriched from mixture saline water samples collected from Chennai (Port of Chennai, salt pan), India. The consortium potently degraded polycyclic aromatic hydrocarbons (> 95%) at 30g/L of sodium chloride concentration in 4 days. The consortium was able to degrade 39 to 45% of different polycyclic hydrocarbons at 60 g/L NaCl concentration. Due to increase in salinity, the percent degradation decreased. To enhance polycyclic aromatic hydrocarbons degradation, yeast extract was added as an additional substrate at 60g/L NaCl concentration. After the addition of yeast extract, the consortium degraded > 74 % of polycyclic aromatic hydrocarbons at 60 g/L NaCl concentration in 4 days. The consortium was also able to degrade PAHs at different concentrations (5, 10, 20, 50 and 100 ppm) with 30 g/L of NaCl concentration. The polycyclic aromatic hydrocarbons degrading halotolerant bacterial consortium consists of three bacterial strains, namely Ochrobactrum sp., Enterobacter cloacae and Stenotrophomonas maltophilia.
KeywordsBiodegradation Polycyclic aromatic hydrocarbons Salinity Halotolerant Bacterial consortium
Unable to display preview. Download preview PDF.
- Agbozu, I. E.; Opuene, K., (2009). Occurrence and Diagenetic Evolution of Perylene in the Sediments of Oginigba Creek, Southern Nigeria. Int. J. Environ. Res., 3(1), 117–120 (4 pages).Google Scholar
- Atlas, R., (1981). Microbial degradation of petroleum hydrocarbons: An environmental perspective. Microbiol. Rev., 45(1), 180–209 (30 pages).Google Scholar
- Gomes, R. C. B.; Nogueira, R.; Oliveira, J. M.; Peixoto, J.; Brito, A. G., (2006). Kinetics of fluorene biodegradation by a mixed culture. Proceedings of the second IASTED International Conference Advanced Technology in the Environmental Field. 6–8 Feb., 2006, Lanzarote, Canary Island, Spain. 84–87.Google Scholar
- Kiyohara, H.; Nagao, K.; Yana, K., (1982). Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates. Appl. Environ. Microbiol., 43(2), 454–457 (4 pages).Google Scholar
- Lee, K. H.; Byeon, S. H., (2010). The biological monitoring of urinary 1hydroxypyrene by PAH exposure among smokers. Int. J. Environ. Res., 4(3), 439–442 (4 pages).Google Scholar
- Mohanan, S., Maruthamuthu, S., Muthukumar, N., Rajasekar, A., Palaniswamy, N., (2007). Biodegradation of Palmarosa oil (Green oil) by Serratia marcescens. Int. J. Environ. Sci. Tech., 4(2), 277–281 (5 pages).Google Scholar
- Nicholson, C. A.; Fathepure, B. Z., (2004). Biodegradation of Benzene by Halophilic and halotolerant bacteria under aerobic conditions. Appl. Microbiol. Biotech., 70(2), 1222–1225 (4 pages).Google Scholar
- Okafor, E. Ch., Opuene, K., (2007). Preliminary assessment of trace metals and polycyclic aromatic hydrocarbons in the sediments. Int. J. Environ. Sci. Tech., 4(2), 233–240 (8 pages).Google Scholar
- Pinyakong, O.; Habe, H.; Supaka, N.; Pinpanichkarn, P.; Juntongjin, K.; Yoshida, T.; Furihata, K.; Nojiri, H.; Yamane. H.; Omori, T., (2000). Identification of novel metabolites in the degradation of phenanthrene by Sphingomonas sp. strain P2. FEMS Microbiol. Lett., 191(1), 115–121 (7 pages).CrossRefGoogle Scholar
- Swannell, R. P.J.; Lee, K.; McDonagh, M., (1996). Field evaluations of marine oil spill bioremediation. Microbiol. Rev., 60(2), 342–365 (24 pages).Google Scholar
- Swannell, R. P. J.; Mitchell, D.; Lethbridge, G.; Jones, D.; Heath, D.; Hagley, M.; Jones, D. M.; Petch, S.; Milne, R.; Croxford, R.; Lee, K., (1999). A field demonstration of the efficiency of bioremediation to treat an oiled shoreline following the Sea Empress incident. Environ. Tech., 20(8), 863–874 (12 pages).CrossRefGoogle Scholar