Assessment of airborne bacteria and fungi in an indoor and outdoor environment
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Airborne indoor and outdoor bacteria and fungi were assessed during the spring season using conventional methods to investigate the enumeration and identification of airborne micro-organisms. This was determined through air quality sampling using the ‘open plate technique’. The air samples were collected during the spring season (March-May) from four different locations. Conventional enumeration of airborne micro-organisms relies on culture-based or microscopic methods. Although a culture-based analysis is most widely used for bio-aerosol, four public places located in urban residential areas were selected for indoor/outdoor air bio-pollutant measurement. The public places included kitchens, classrooms, recreational areas, laboratories. Public parks are an important facility associated with the environmental exposure of children. Cultivation and total microscopic enumeration methods were employed for the sample analysis. 26 groups of bacteria and fungi, either of human or environmental origin were detected. Environmental agents generally predominated while significantly higher counts were detected as the level of hygiene or standard of housing dropped. Seven genera of fungi, mainly members of the genus Aspergillum, were isolated from all residents. Bacteria shows higher growth numbers as opposed to the slow growing fungi. Sample collection and pretreatment, determination techniques and performance results are summarized and discussed.
KeywordsAspergillum Bioaerosols Conventional methods Microorgansim
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- Cox, C. S.; Wathes, C. M., (1995). Bioaerosols handbook. NY: Lewis Publishers.Google Scholar
- Cuthbertson, A. G. S.; Blackburn, L. F.; Northing, P.; Luo, W.; Cannon, R. J. C.; Walters, K. F. A., (2010).Chemical compatibility testing of the entomopathogenic fungus Lecanicillium muscarium to control Bemisia tabaci in glasshouse environment. Int. J. Environ. Sci. Tech., 7(2), 405–409 (5 pages).Google Scholar
- Dhanasekaran, D.; Thajuddin, N.; Rashmi, M.; Deepika, T. L.; Gunasekaran, M., (2009). Screening of biofouling activity in marine bacterial isolate from ship hull. Int. J. Environ. Sci. Tech., 6(2), 197–202 (8 pages).Google Scholar
- Gorny, R. L., (2004). Filamentous microorganisms and their fragments in indoor air: A review. Ann. Agric. Environ. Med., 11, 185–197 (12 pages).Google Scholar
- Jensen, P. A.; Schafer, M. P., (1998). Sampling and characterization of bioaerosols. NIOSH manual of analytical methods.Google Scholar
- Madukasi, E. I.; Dai, X.; He, C.; Zhou, J., (2010). Potentials of phototrophic bacteria in treating pharmaceutical wastewater. Int. J. Environ. Sci. Tech., 7(1), 165–174 (10 pages).Google Scholar
- Maeir, R. M.; Pepper, J. L.; Gerba, P. C., (2002). Environmental microbiology. Canada, Academic Press.Google Scholar
- Okafor, E. Ch.; Opuene, K., (2007). Preliminary assessment of trace metals and polycyclic aromatic hydrocarbons in the sediments. Int. J. Environ. Sci. Tech., 4(2), 233–240 (8 pages).Google Scholar
- Stetzenbach, L. D., (2007). Introduction to aerobiology. Hurst, C. J.; Crawford, R. L.; Garland, J. L.; Lipson, D. A.; Mills, A. L.; Stetzenbach, L. D., (Eds.). Manual of environmental microbiology, ASM Press, Washington D.C., 925–938 (14 pages).Google Scholar