Assessment of airborne bacteria and fungi in an indoor and outdoor environment

  • M. F. YassinEmail author
  • S. Almouqatea


Airborne indoor and outdoor bacteria and fungi were assessed during the spring season using conventional methods to investigate the enumeration and identification of airborne micro-organisms. This was determined through air quality sampling using the ‘open plate technique’. The air samples were collected during the spring season (March-May) from four different locations. Conventional enumeration of airborne micro-organisms relies on culture-based or microscopic methods. Although a culture-based analysis is most widely used for bio-aerosol, four public places located in urban residential areas were selected for indoor/outdoor air bio-pollutant measurement. The public places included kitchens, classrooms, recreational areas, laboratories. Public parks are an important facility associated with the environmental exposure of children. Cultivation and total microscopic enumeration methods were employed for the sample analysis. 26 groups of bacteria and fungi, either of human or environmental origin were detected. Environmental agents generally predominated while significantly higher counts were detected as the level of hygiene or standard of housing dropped. Seven genera of fungi, mainly members of the genus Aspergillum, were isolated from all residents. Bacteria shows higher growth numbers as opposed to the slow growing fungi. Sample collection and pretreatment, determination techniques and performance results are summarized and discussed.


Aspergillum Bioaerosols Conventional methods Microorgansim 


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  1. Abdul Hameed, A. A.; Khoder, M. I.; Yuosra, S.; Osman, A. M.; Ghanem, S., (2009). Diurnal distribution of airborne bactria and fungi in the atmosphere of Helwan area, Egypt. Sci. Total Environ., 407(24), 6217–6222 (6 pages).CrossRefGoogle Scholar
  2. Banerjee, D., (2008). Study of precipitation chemistry over an industrial city. Int. J. Environ. Sci. Tech., 5(3), 331–338 (8 pages).CrossRefGoogle Scholar
  3. Cox, C. S.; Wathes, C. M., (1995). Bioaerosols handbook. NY: Lewis Publishers.Google Scholar
  4. Cuthbertson, A. G. S.; Blackburn, L. F.; Northing, P.; Luo, W.; Cannon, R. J. C.; Walters, K. F. A., (2010).Chemical compatibility testing of the entomopathogenic fungus Lecanicillium muscarium to control Bemisia tabaci in glasshouse environment. Int. J. Environ. Sci. Tech., 7(2), 405–409 (5 pages).Google Scholar
  5. Dhanasekaran, D.; Thajuddin, N.; Rashmi, M.; Deepika, T. L.; Gunasekaran, M., (2009). Screening of biofouling activity in marine bacterial isolate from ship hull. Int. J. Environ. Sci. Tech., 6(2), 197–202 (8 pages).Google Scholar
  6. Fracchia, L.; Pietronave, S.; Rinaldi, M.; Martinotti, M. G., (2006). The assessment of airborne bacterial contamination in three composting plants revealed site-related biological hazard and seasonal variations. J. Appl. Microbiol., 100(5), 973–984 (12 pages).CrossRefGoogle Scholar
  7. Gorny, R. L.; Reponen, T.; Willeke, K.; Schmechel, D.; Robine, E.; Boissier, M.; Grinshpun, S. A., (2002). Fungal fragments as indoor air biocontaminants. Appl. Environ. Microbiol., 68(7), 3522–3531 (10 pages).CrossRefGoogle Scholar
  8. Gorny, R. L., (2004). Filamentous microorganisms and their fragments in indoor air: A review. Ann. Agric. Environ. Med., 11, 185–197 (12 pages).Google Scholar
  9. Howard-Reed, C.; Wallace, L. A.; Emmerich, S. J., (2003). Effect of ventilation systems and air filters on decay rates of particles by indoor sources in an occupied townhouse. Atmos. Environ., 37(38), 5295–5306 (12 pages).CrossRefGoogle Scholar
  10. Jamriska, M., (2000). Effect of ventilation and filtration on submicrometer particles in an indoor environment. Indoor Air, 10(1), 19–26 (8 pages).CrossRefGoogle Scholar
  11. Jensen, P. A.; Schafer, M. P., (1998). Sampling and characterization of bioaerosols. NIOSH manual of analytical methods.Google Scholar
  12. Madukasi, E. I.; Dai, X.; He, C.; Zhou, J., (2010). Potentials of phototrophic bacteria in treating pharmaceutical wastewater. Int. J. Environ. Sci. Tech., 7(1), 165–174 (10 pages).Google Scholar
  13. Maeir, R. M.; Pepper, J. L.; Gerba, P. C., (2002). Environmental microbiology. Canada, Academic Press.Google Scholar
  14. Nkwocha, E. E.; Egejuru, R. O., (2008). Effects of industrial air pollution on the respiratory health of children. Int. J. Environ. Sci. Tech., 5(4), 509–516 (8 pages).CrossRefGoogle Scholar
  15. Okafor, E. Ch.; Opuene, K., (2007). Preliminary assessment of trace metals and polycyclic aromatic hydrocarbons in the sediments. Int. J. Environ. Sci. Tech., 4(2), 233–240 (8 pages).Google Scholar
  16. Pasanen, A. L.; Lappalainen, S.; Pasanen, P., (1996). Volatile organic metabolites associated with some toxic fungi and their mycotoxins. Analyst, 121, 1949–1953 (5 pages).CrossRefGoogle Scholar
  17. Stetzenbach, L. D., (2007). Introduction to aerobiology. Hurst, C. J.; Crawford, R. L.; Garland, J. L.; Lipson, D. A.; Mills, A. L.; Stetzenbach, L. D., (Eds.). Manual of environmental microbiology, ASM Press, Washington D.C., 925–938 (14 pages).Google Scholar
  18. Velmurugan, N.; Chun, S. S.; Han, S. S.; Lee, Y. S., (2008). Characterization of chikusaku-eki and mokusakueki and its inhibitory effect on sapstaining fungal growth in laboratory scale. Int. J. Environ. Sci. Tech., 6(1), 13–22 (10 pages).CrossRefGoogle Scholar
  19. Venkateswaran, K.; Hattori, N.; La Duc, M. T.; Kern, R., (2003). ATP as a biomarker of viable microorganisms in clean-room facilities. J. Microbiol. Method., 52(3), 367–377 (11 pages).CrossRefGoogle Scholar
  20. Wallace, L. A., Emmerich, S. J., Howard-Reed, C., (2004). Effect of central fans and in-duct filters on deposition rates of ultrafine and fine particles in an occupied townhouse. Atmospher. Environ., 38(3), 405–413 (9 pages).CrossRefGoogle Scholar

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© Islamic Azad University 2010

Authors and Affiliations

  1. 1.Department of Environmental Technology and Management, College for WomenKuwait UniversityKuwaitKuwait
  2. 2.Faculty of EngineeringAssiut UniversityAssiutEgypt
  3. 3.Kuwait Institute for Scientific ResearchSafatKuwait

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