A procedure for in vitro propagation of pharmaceutically valuable varieties of Caralluma adscendens from nodal explant, is described. The highest shoot multiplication with 80% frequency was achieved within one month on Murashige and Skoog’s medium supplemented with 8.87 μM BA. Shoot multiplication occurred in subsequent subcultures in culture bottles on MS medium. Regenerated shoots were rooted on half strength MS medium supplemented with NAA (0.54 μM) in all the three varieties. The rooted plants were hardened for establishment in soil.
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6 — benzyl adenine
Indole acetic acid
Indole butyric acid
Murashige and Skoog
Naphthalene acetic acid
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Aruna, V., Kiranmai, C., Karuppusamy, S. et al. Micropropagation of Three Varieties of Caralluma adscendens via Nodal Explants. J. Plant Biochem. Biotechnol. 18, 121–123 (2009). https://doi.org/10.1007/BF03263309
- Caralluma adscendens
- nodal explant
- benzyl adenine
- medicinal plant