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Micropropagation of Three Varieties of Caralluma adscendens via Nodal Explants

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Abstract

A procedure for in vitro propagation of pharmaceutically valuable varieties of Caralluma adscendens from nodal explant, is described. The highest shoot multiplication with 80% frequency was achieved within one month on Murashige and Skoog’s medium supplemented with 8.87 μM BA. Shoot multiplication occurred in subsequent subcultures in culture bottles on MS medium. Regenerated shoots were rooted on half strength MS medium supplemented with NAA (0.54 μM) in all the three varieties. The rooted plants were hardened for establishment in soil.

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Abbreviations

AC:

activated charcoal

BA:

6 — benzyl adenine

CA:

citric acid

CH:

casein hydrolysate

IAA:

Indole acetic acid

IBA:

Indole butyric acid

2iP:

6-γ,γ-dimethylallylaminopurine

KN:

kinetin

MS:

Murashige and Skoog

NAA:

Naphthalene acetic acid

PVP:

polyvinyl pyrrolidine

YE:

yeast extract

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Author information

Correspondence to T. Pullaiah.

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Aruna, V., Kiranmai, C., Karuppusamy, S. et al. Micropropagation of Three Varieties of Caralluma adscendens via Nodal Explants. J. Plant Biochem. Biotechnol. 18, 121–123 (2009). https://doi.org/10.1007/BF03263309

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Key words

  • Caralluma adscendens
  • nodal explant
  • benzyl adenine
  • NAA
  • medicinal plant