Abstract
Selecting the explant genotypes is crucial step in in vitro culture and Agrobacterium-mediated transformation system due to its host range specificity. Immature embryos of five winter and three spring wheat (Triticum aestivum L) cultivars were evaluated for tissue culture response in three callus initiation media. MS medium containing 2,4-0 (2 mg ml-1) plus B5 vitamins (MSB5), MS medium containing 2,4-0 (1 mg ml-1) with no vitamins (MS1GC) or MS medium containing picloram (2.2 mg ml-1) and 2,4-0 (0.5 mg ml-1) plus MS vitamins (CM4C) were used for callus initiation. Percentage of callus induction varied widely with the genotype and initiation medium used, with values ranging from 5.7% to 100%. Embryogenic capacity of genotypes was evaluated by number of somatic embryos formed from cultured immature embryos. Bob White (spring) and NE92458 (winter) were equal and most embryogenic; Pronghorn and 2137 (both winter) were the poorest. CM4C medium was found to be the best medium for initiating embryogenic callus among three culture media tested. A standard regeneration procedure was used. The genotypes with the highest regeneration efficiencies were Bob White, Fielder and NE92458, (1.8, 1.4 and 1.6 plantslexplant, respectively).
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References
Ahloowalia BS, Crop Sci, 22 (1982) 405.
Sears RG & Deckard EL, Crop Sci, 22 (1982) 546.
Ozias-Akins P, & Vasil IK, Protoplasma, 110 (1982) 95.
Weeks JT, Anderson OD & Blechl AE, Plant Physiol, 102 (1993) 1077.
Haliloglu K & Baenziger PS, Cereal Res Commun, 31 (2003) 9.
Mathias RJ & Simpson ES, Plant Cell Tiss Org Cult, 7 (1986) 31.
Fennell S, Bohorova N, van Ginkel M, Crossa J & Hoisington D, Theor Appl Genet, 92 (1995) 163.
Elena EB & Ginzo HD, J Plant Physiol, 132 (1988) 600.
He DG, Yang YM & Scott KJ, Plant Sci, 57 (1988) 225.
Carman JG, Jefferson NE & Campbell WF, Plant Cell Tiss Organ Cult, 10 (1998) 101.
Cheng M, Joyce E, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW & Wan Y, Plant Physiol, 115 (1997) 971.
Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.
Gamborg OL, Miller RA & Ojima K, Exp Cell Res, 50 (1968) 151.
Waller RA & Duncan DB, J Am Stat Assoc, 64 (1969) 1484.
SAS Institute Inc. (1996) SAS Institute, Cary, NC.
Zhou H, Arrowsmith JW, Fromm ME, Hironaka CM, Taylor ML, Rodriguez, D, Pajeau ME, Brown SM, Santino CG & Fry JE, Plant Cell Rep, 15 (1995) 159.
He DG, Yang TM, Bertram J & Scott KJ, Plant Sci, 68 (1990) 103.
Maddock SE, Lancaster VA, Risiott R & Franklin J, Exp Bot, 34 (1987) 915.
Redway FA, Vasil V & Vasill K, Plant Cell Rep, 8 (1990) 714.
Maheshwary N, Rajyalakshmi K, Baweja K, Dhir SK, Chowdhry CN & Maheshwary SC, Crfit Rev Plant Sci, 14 (1995) 149.
Boulton MI, Steinkellner H, Donson J, Markham PG, King DI & Davis KW, J Gen Viral, 70 (1989) 2309.
Mooney PA, Goodwin PB, Dennis ES & Liewellyn DJ, Plant Cell Tiss Org Cult, 25 (1991) 209.
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Haliloqlu, K., Baenziger, P.S. Screening Wheat Genotypes for High Callus Induction and Regeneration Capability from Immature Embryo Cultures. J. Plant Biochem. Biotechnol. 14, 155–160 (2005). https://doi.org/10.1007/BF03263244
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DOI: https://doi.org/10.1007/BF03263244