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Efficient Plant Regeneration via Shoot Tip Explant in Jatropha curcas L

Abstract

An efficient regeneration method via shoot tip explant has been developed for Jatropha curcas, which is a medicinally as well as economically important plant. Shoot tips were proliferated on MS medium incorporated with BAP (2.0 mg l−1) and IAA (0.5 mg l−1) along with adenine sulphate, glutamine and activated charcoal. In vitro produced shoots were induced to root on IBA (0.5–5.0 mg l−1) added to half strength MS medium. The highest frequency of root induction was on the medium with 3.0 mg l−1 IBA. Regenerated plantlets were successfully transferred to field after initial acclimatization.

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Abbreviations

BAP:

6-benzylamino purine

IBA:

Indole-3-butyric acid

MS:

Murashige & Skoog’s

SE:

Standard error

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Author information

Correspondence to Shilpa Rajore.

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Rajore, S., Batra, A. Efficient Plant Regeneration via Shoot Tip Explant in Jatropha curcas L. J. Plant Biochem. Biotechnol. 14, 73–75 (2005). https://doi.org/10.1007/BF03263231

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Key words

  • Jatropha curcas
  • shoot tip
  • regeneration