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In Vitro Clonal Propagation of Curcuma caesia Roxb and Curcuma zedoaria Rosc from Rhizome Bud Explants

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Abstract

Two species of Curcuma (C. caesia and C. zedoaria) have been propagated through tissue culture using rhizome bud explant. The best response for shoot multiplication was obtained on MS basal medium supplemented with 4 mg l−1 BAP and 1.5 mg l−1 NAA for C. caesia (3.5 ± 0.79 shoots per explant) and 1 mg l−1 BAP + 0.5 mg l−1 NAA for C. zedoaria (4.5 ± 0.15 shoots per explant). A maximum of 9.2 ± 0.15 and 8.9 ± 0.09 roots per explant were obtained for C. caesia and C. zedoaria, respectively when MS was supplemented with 0.5 mg l−1 IAA. The rooted plants could be established in soil.

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Abbreviations

BAP:

6-benzylaminopurine

NAA:

α-naphthalene acetic acid

Kn:

kinetin

IAA:

Indole-3-acetic acid

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Authors and Affiliations

  1. Department of Biotechnology, University of Gauhati, Guwahati, 781 014, Assam, India

    Raju Bharalee, Anita Das & M. C. Kalita

Authors
  1. Raju Bharalee
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  2. Anita Das
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  3. M. C. Kalita
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Corresponding author

Correspondence to M. C. Kalita.

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Bharalee, R., Das, A. & Kalita, M.C. In Vitro Clonal Propagation of Curcuma caesia Roxb and Curcuma zedoaria Rosc from Rhizome Bud Explants. J. Plant Biochem. Biotechnol. 14, 61–63 (2005). https://doi.org/10.1007/BF03263228

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  • DOI: https://doi.org/10.1007/BF03263228

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