Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Isozyme Profile During Somatic Embryogenesis and In Vitro Flowering of Bambusa vulgaris

  • 3 Citations

Abstract

Peroxidase and esterase isozymes were investigated during plant regeneration via somatic embryogenesis in Bambusa vulgaris, The transition of non-embryogenic calli to embryogenic calli, somatic embryo development, germination and subsequent flowering of somatic embryo derived shoots were associated with selective expression or repression of isoforms of peroxidase and esterase. Non-embryogenic callus showed six peroxidase and four esterase bands. During somatic embryogenesis and germination of somatic embryos, some bands were suppressed and new isoforms of peroxidase and esterase appeared. During flowering, in addition to four peroxidase bands, a new unique esterase band ‘a’ appeared. Each developmental stage was thus associated with a definite isozyme profile.

This is a preview of subscription content, log in to check access.

References

  1. 1

    Scandalios JG, Ann Rev Plant Physiol, 25 (1974) 225.

  2. 2

    Scandalios JG & Sorenson JC, In Applied and fundamental aspect of plant cell, tissue, and organ culture (J Reinert, YPS Bajaj, Editors), Springer-Verlag, Berlin (1977) p 719.

  3. 3

    Kay EL & Basile OV, Plant Physiol, 84 (1987) 99.

  4. 4

    Fransz PF, deFuijter NCA & Schel JHN, Plant Cell Reports, 8 (1989) 67.

  5. 5

    Bapat SA, Rawal SK & Mascarenhas AF, Plant Sci, 82 (1992) 235.

  6. 6

    Moxi L, Toshinori ABE & Futsuhara Y, Japan J Breed, 42 (1992) 535.

  7. 7

    Mehta U, Ramanuja Rao IV & MohanRam HY, In Proc 8th Int Cong Plant Tissue Cell Culture (1982) P 109.

  8. 8

    Rao U, Ramanuja Rao IV & Narang V, Plant Cell Reports, 4 (1985) 191.

  9. 9

    Mascarenhas AF, Khuspe SS, Nadgauda RS & Khan BBM, In Genetic manipulation of woody plants (J Hanover, O Keathley, Editors), Plenum Press, New York (1988).

  10. 10

    Woods SH, Phillips GC, Woods JE & Collins GB, Plant Cell Reports, 11 (1992) 257.

  11. 11

    Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.

  12. 12

    Wetter L & Oyck J, In Handbook of plant cell culture, Vol. 1 (DA Evan, WR Sharp, PV Ammirato, Y Yamada, Editors), MacMillan, New York (1983) p 607.

  13. 13

    Eduardo V, In Plant genetics and breeding, Part-A, (SO Tanksely, TO Orton, Editors), Elsevier, Amsterdam (1983) p 469.

  14. 14

    Pedersen S & Andersen SB, Plant Sci, 91 (1993) 75.

  15. 15

    Carmon JG, In Vitro Cell Dev Bioi, 26 (1990) 746.

  16. 16

    Vasil V, Vasil IK & Lu CY, Amer J Bot, 71 (1984) 158.

  17. 17

    Hahne G, Mayer JE & Lorz H, Plant Sci, 55 (1988) 267.

Download references

Author information

Correspondence to Gyana R. Rout.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rout, G.R., Das, P. Isozyme Profile During Somatic Embryogenesis and In Vitro Flowering of Bambusa vulgaris . J. Plant Biochem. Biotechnol. 4, 43–45 (1995). https://doi.org/10.1007/BF03262950

Download citation

Key words

  • Bambusa vulgaris
  • esterase
  • peroxidase
  • isozyme
  • somatic embryogenesis
  • PAGE