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Properties of ATP-dependent Phosphofructokinase from Endosperm of Developing Wheat (Triticum aestivum L.) Grains

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Abstract

ATP-dependent phosphofructokinase (ATP:D-fructose S-phosphate, 1-phosphotransferase, EC 2.7.1.11, PFK) from endosperm of developing wheat grains was purified to apparent homogeneity with about 45% recovery using ammonium sulfate fractionation, ion-exchange chromatography on DEAE-cellulose and gel filtration through Sepharose CL-SB and Sephadex G-200. The purified enzyme with a molecular weight of about 182 kD, was a heterotetramer with subunit molecular weights ranging between 20 and 80 kD. The enzyme exhibited maximum activity at pH 7.9 and was highly specific for its substrates. The enzyme had absolute requirement for Mg2+. At pH 7.9, the Km values as determined by Lineweaver-Burk plots were 1.43 and 0.70 mM, respectively for fru-S-P and ATP. Fru-2, S-P2 had no effect on the activity of the enzyme. The enzyme was inhibited strongly by citrate, ADP, 3-PGA and PEP with Ki values of 2.40, 1.75, 2.10 and 0.80 mM, respectively. Citrate and PEP inhibited the enzyme competitively with respect to both fru-S-P and ATP. ADP and 3-PGA inhibited the enzyme non-competitively and competitively, respectively with respect to fru-S-P and in a mixed manner with respect to ATP. Hill plot values indicated co-operative interaction of citrate, 3-PGA and PEP with the enzyme.

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Correspondence to Randhir Singh.

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Mahajan, R., Singh, R. Properties of ATP-dependent Phosphofructokinase from Endosperm of Developing Wheat (Triticum aestivum L.) Grains. J. Plant Biochem. Biotechnol. 1, 45–48 (1992). https://doi.org/10.1007/BF03262894

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Key words

  • endosperm
  • phosphofructokinase
  • properties
  • Triticum aestivum
  • wheat