European Archives of Paediatric Dentistry

, Volume 11, Issue 1, pp 26–31 | Cite as

Microleakage of three different sealants on sound and questionable occlusal surfaces of permanent molars: An in vitro study

  • M. G. Michalaki
  • C. J. OulisEmail author
  • P. Lagouvardos


AIM: This was to evaluate the microleakage of 3 different sealants, applied on sound and questionably carious occlusal surfaces with and without a bonding agent. METHODS: A total of 120 human molars were selected, photographed with a digital video microscope, and assigned by 3 independent examiners, according to the criteria of ICDAS II, in 2 groups of 60 teeth each. Group A: teeth with deep, clear, sound occlusal surfaces (ICDAS II, code 0). Group B: teeth with questionable occlusal surfaces, having deep, stained pits and fissures with probable incipient, but non-cavitated, carious lesions (ICDAS II, codes 1 and 2). Each group was divided into 2 subgroups of 30 teeth each (bonding or no bonding) and then into 3 subgroups of 10 teeth each according to the type of sealant used: one conventional (Conseal®) and 2 fluoridated (Conseal F® and Teethmate F®). After the application of the bonding agent and the sealant to the appropriate teeth, all specimens were subjected to thermal cycling and immersed in a 10% methylene blue dye solution for 4 hours. Average and summed microleakage for each sample were estimated from dye penetration scores on 3 mesiodistal sections of the tooth across the sealed occlusal surface. Nonparametric Friedman’s 2-way ANOVA by ranks and Conover-Inman pair wise comparisons were used for differences at the 0.05 level of significance. RESULTS: According to Friedman’s 2-way ANOVA by ranks analysis, although there were no significant differences between the different sealants (χ2= 0.048, df = 2, P =.976), there were significant differences between the sound and questionably carious occlusal surfaces (χ2 = 24, df = 3,.000). Conover-Inman pair wise comparisons showed no differences between the groups using and not using bonding agents, on sound (SNB-SWB, P =.4561) or questionable occlusal surfaces (QNB-QWB, P =.0842). CONCLUSIONS: Sealant microleakage on questionably carious occlusal surfaces was statistically significantly higher than that of sound occlusal surfaces. Using a bonding agent or fluoridated FS did not influence microleakage significantly, either on sound or on questionable fissured surfaces.

Key words

fluoridated sealant questionable surfaces bonding agent microleakage 


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Copyright information

© Adis International 2010

Authors and Affiliations

  • M. G. Michalaki
    • 1
  • C. J. Oulis
    • 1
    Email author
  • P. Lagouvardos
    • 2
  1. 1.Department of Paediatric Dentistry, School of DentistryUniversity of AthensHalandri, AthensGreece
  2. 2.Dept. of Operative Dentistry, School of DentistryUniversity of AthensGreece

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