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Molecular Diagnosis

, Volume 8, Issue 1, pp 11–16 | Cite as

Enteroviral Infection in Greek AIDS Patients

  • Dimitrios Papaventsis
  • Panayotis MarkoulatosEmail author
  • Nikolaos Mangafas
  • Marios Lazanas
  • Stamatina Levidiotou-Stefanou
Original Research Article
  • 15 Downloads

Abstract

Objective: Prolonged intestinal replication of polioviruses has not previously been studied in Greek AIDS patients. The objective of our study was to estimate the prevalence of enteroviral infections in this population.

Methods: Nineteen stool samples were investigated from 19 different patients. Collection took place at the Hellenic Red Cross Hospital, Athens, Greece, between August and October 2002. Samples were processed as follows: virus isolation was attempted by cell culture using three different cell lines (human epidermoid carcinoma [Hep]-2, rabdomyosarcoma [RD], and mouse cells genetically modified in order to express the polio virus receptor in their cell surface [L20b]). An enterovirus-specific reverse transcription (RT)-PCR was then applied. Finally, seroneutralization tests were performed on 11 blood samples taken from a number of the patients who had supplied stool samples.

Results: Samples were negative for enterovirus detection of any serotype on all cell lines. No cytopathic effect was observed. Enterovirus-specific RT-PCR assays were also negative for the detection of enteroviral RNA. Seroneutralization revealed relatively high antibody titers against poliovirus 1 and 2 in three of the eleven blood samples.

Conclusions: Greek AIDS patients are not vulnerable to enteroviral infections and do not constitute a potential reservoir of poliovirus-prolonged excretion in Greece.

Keywords

Human Immunodeficiency Virus Aseptic Meningitis Oral Poliovirus Vaccine Enteroviral Infection Paralytic Poliomyelitis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We gratefully acknowledge Aikaterini Moustakli, nurse at the Infectious Diseases Department, Red Cross General Hospital, Athens, for her essential and precious help during sample collection and Eleni Afentaki, technologist at the Department of Virology, Hellenic Pasteur Institute, Athens, for her technical support.

This work was funded by the Délégation Génerale au Réseau International des Instituts Pasteur et Instituts assosiés (AC 99 Entérovirus & AC 02 Vaccin Polio Oral).

The authors have no conflicts of interest directly relevant to the content of this study.

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Copyright information

© Adis Data Information BV 2004

Authors and Affiliations

  • Dimitrios Papaventsis
    • 1
    • 2
    • 3
  • Panayotis Markoulatos
    • 4
    Email author
  • Nikolaos Mangafas
    • 2
  • Marios Lazanas
    • 2
  • Stamatina Levidiotou-Stefanou
    • 3
  1. 1.Department of VirologyHellenic Pasteur InstituteAthensGreece
  2. 2.Infectious Diseases DepartmentRed Cross General HospitalAthensGreece
  3. 3.Microbiology Laboratory, Medical SchoolUniversity of IoanninaIoanninaGreece
  4. 4.Microbiology-Virology Laboratory, Department of Biochemistry and BiotechnologyUniversity of ThessalyLarissaGreece

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