Molecular Diagnosis & Therapy

, Volume 10, Issue 1, pp 41–47 | Cite as

Detection of Circulating Tumor Cells in Peripheral Blood of Breast Cancer Patients During or After Therapy Using a Multigene Real-Time RT-PCR Assay

  • Barbara K. Zehentner
  • Heather Secrist
  • Dawn C. Hayes
  • Xinqun Zhang
  • Richard C. Ostenson
  • Steven Loop
  • Gary Goodman
  • Raymond L. Houghton
  • David H. Persing
Original Research Article

Abstract

Aim: To evaluate the utility of a multigene real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay to detect circulating tumor cells in peripheral blood specimens of breast cancer patients during or after treatment.

Method: Using this assay, peripheral blood samples were analyzed for expression levels of mammaglobin and three complementary transcribed breast cancer-specific genes: B305D, γ-aminobutyrate type A receptor π subunit (GABA π; GABRP), and B726P. We examined 172 blood specimens from 82 breast cancer patients during or after therapy for the presence of circulating tumor cells using the multigene real-time RT-PCR assay.

Results: In 63.4% of the blood samples, a positive signal for mammaglobin and/or three breast cancer-associated gene transcripts was detected. Of breast cancer patients, 75.6% had at least one positive blood sample. Blood specimens from 51 of 53 healthy female volunteers tested negative in the assay whereas two samples had a low expression signal. In addition, three patients were monitored for more than a year during their adjuvant therapy treatment.

Conclusion: This assay could be a valuable tool for monitoring breast cancer patients during and after therapy.

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Copyright information

© Adis Data Information BV 2006

Authors and Affiliations

  • Barbara K. Zehentner
    • 1
  • Heather Secrist
    • 2
  • Dawn C. Hayes
    • 2
  • Xinqun Zhang
    • 2
  • Richard C. Ostenson
    • 3
  • Steven Loop
    • 3
  • Gary Goodman
    • 4
  • Raymond L. Houghton
    • 5
  • David H. Persing
    • 2
  1. 1.HematoLogics Inc.SeattleUSA
  2. 2.Corixa CorporationSeattleUSA
  3. 3.Good Samaritan Cancer CenterPuyallupUSA
  4. 4.Swedish Medical CenterSeattleUSA
  5. 5.InBios International Inc.SeattleUSA
  6. 6.GSK-Bio-Seattle (formerly Corixa Corporation)SeattleUSA

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