The pharmacokinetics of a fibrin adhesive agent applied to the rat lung

Abstract

Purpose: Although fibrin adhesive agents are frequently applied in the clinical setting, their pharmacokinetics in vivo remain to be clarified. We examined the pharmacokinetics of a fibrin adhesive agent applied to the rat lung. Material and methods: Male Sprague Dawley rats were used. Under general anesthesia, left thoracotomy was performed, and the left lung was incised about 1 cm length and 1 mm depth. This incision was sutured with 9-0 nylon, and a fibrin adhesive agent containing¹²⁵I-labeled fibrinogen was applied. On days 1, 3, 7 and 14 after the operation, the left lung, right lung, liver and kidneys were collected. The tissue distribution of radioactivity was examined by determining the¹²⁵I levels in each organ as well as calculating the tissue levels of radioactivity. Results: The tissue distribution of radioactivity in the left lung was significantly higher than those in other organs on days 1 and 3. The tissue levels of radioactivity in the left lung was significantly higher than those in other organs on days 1, 3 and 7. Each value rapidly decreased after day 7. Conclusion: A fibrin adhesive agent applied to the lung significantly remained at a high level through the inflammatory and proliferative phases followed by a prompt decrease before the phase of cicatrization. Therefore it is considered that a fibrin adhesive agent applied to the lung is satisfactory for the healing of wounds.