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Stress responses analysis of As(III), Cd and Pb inEscherichia coli

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Abstract

In this study, it was analyzed specific stress responses caused by heavy metals such as sodium arsenite (As(III)), cadmium nitrate (Cd) and led nitrate (Pb) using four different stress responsive genes inEscherichia coli, i.e.fabA, grpE, katG andrecA. The expression levels of these genes were quantified by semi-quantitative reverse transcription-PCR. It was found that four genes have shown different responsive patterns whenE. coli cultures were under stressful conditions caused by As(III), Cd and Pb, respectively. Therefore, in this study, the stress responsive effects caused by these heavy metals and the extent of each stress response can be analyzed using the expression levels and patterns of different stress responsive genes.

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References

  1. 1.

    Rainbow, P. S. & White, S. L. Comparative strategies of heavy metal accumulation by crustaceans: Zinc, copper and cadmium in a decapod, an amphipod and a barnacle.Hydrobiologia 174, 245–262 (1989).

  2. 2.

    Simmons, P. T. & Potier, C. J. Toxicogenomics: The new frontier in risk analysis.Carcinogenesis 23, 903–905 (2002).

  3. 3.

    Mager, W. H. & De Kruijff, A. J. Stress-induced transcriptional activation.Microbial Rev 59, 506–531 (1995).

  4. 4.

    Bromberg, R., George, S. M. & Peck, M. Oxygen sensitivity of heated cells of Escherichia coli O157: H7.J Appl Microbiol 85, 231–237 (1998).

  5. 5.

    Choi, S. H. & Gu, M. B. Phenolic toxicity-Detection and classification through the use of a recombinant bioluminescent Escherichia coli.Environ Toxicol Chem 20, 248–255 (2001).

  6. 6.

    Vollmer, A. C., Belkin, S., Smulski, D. R., VanDyk, T. K. & LaRossa, R. A. Detection of DNA damage by use of Escherichia coli Carrying recA::lux, uvrA::lux, or alkA::lux Reporter Plamids.Appl Env Microbial 63, 2566–2571 (1997).

  7. 7.

    Min, J., Kim, E. J., LaRossa, R. A. & Gu, M. B. Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents.Mutat Res 442, 61–68 (1999).

  8. 8.

    Peraza, M. A., Ayala-Fierro, F., Barber, D. S., Casarez, E. & Rael, L. T. Effects of micronutrients on metal toxicity.Environ Health Perspect 106, 203–216 (1998).

  9. 9.

    Sillanpää, M. & Oikari, A. Assessing the impact of complexation by EDTA and DTPA on heavy metal toxicity using microtox bioassay.Chemosphere 32, 1485–1497 (1996).

  10. 10.

    Sorvari, J. & Sillanpää, M. Influence of metal complex formation on heavy metal and free EDTA and DTPA acute toxicity determined by Daphnia magna.Chemosphere 33, 1119–1127 (1996).

  11. 11.

    Klein, D. Quantification using real-time PCR technology: Applications and limitations.Trends Mol Med 8, 257–260 (2002).

  12. 12.

    Demple, B. Regulation of bacterial oxidative stress genes.Annu Rev Genet 25, 315–337 (1991).

  13. 13.

    Chiorcea-Paquim, A. M., Corduneanu, O., Oliveira, S. C. B., Diculescu, V. C. & Oliveria-Brett, A. M. Electrochemical and AFM evaluation of hazard compounds-DNA interaction.Electrochimica Acta 54, 1978–1985 (2009).

  14. 14.

    Kasprzak, K. S. Oxidative DNA and protein damage in metal-induced toxicity and carcinogenesis.Free Radic Biol Med 32, 958–967 (2002).

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Correspondence to Yang-Hoon Kim or Jiho Min.

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Lim, E., Le, T., Lee, S. et al. Stress responses analysis of As(III), Cd and Pb inEscherichia coli . Toxicol. Environ. Health. Sci. 1, 192–195 (2009). https://doi.org/10.1007/BF03216485

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Keywords

  • Heavy metals
  • E. coli
  • Stress responsive genes
  • Gene expression patterns