Pulse Amplitude Modulated (PAM) fluorometry is a sensitive and rapid method used to assess toxic effect of pollutants in plants as well as algae. This study evaluates the difference in sensitivity of two marine macroalgae,Ulva pertusa andEcklonia cava, to copper. The photosynthetic efficiency of the algae was measured as the ratio of variable to maximal chlorophyll fluorescence (Fv/Fm) as well as maximum electron transport rate (ETRmax). The algae were exposed to 0.125–1 mg/L of copper and their physiology was studied every 24 h for a period of 4 days. Increase in copper concentration caused proportional decrease in the photosynthetic efficiency, particularly ETRmax, of both the species studied. Moreover,U. pertusa proved to be more sensitive to copper thanE. cava, thus implying its use as a toxicity biotest battery.
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Kumar, K.S., Han, Y., Choo, K. et al. Chlorophyll fluorescence based copper toxicity assessment of two algal species. Toxicol. Environ. Health. Sci. 1, 17–23 (2009). https://doi.org/10.1007/BF03216459
- Chlorophylla fluorescence
- Ecklonia cava
- Ulva pertusa