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Genes associated with long-chain omega-3 fatty acids in bovine skeletal muscle

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Abstract

Long-chain omega-3 fatty acids (n-3 FAs) influence meat tenderness, juiciness, and flavor, and are beneficial to human health. The percentage of long-chain n-3 FAs in total FAs is termed the omega-3 index (O3I). It is thus of great interest to favor rising this index in bovine skeletal muscle, to obtain healthier, tastier, and more nutritive meat. This study was aimed to detect transcriptomic variations related to O3I in muscles in 15-month-old males of 4 Spanish cattle breeds raised under the same conditions. Through the analysis of extreme O3I phenotypes, 3 genes of interest (AANAT, UCP2 andAHA1) were identified.AANAT andUCP2 were strongly up-regulated, whileAHA1 was repressed in animals with a high O3I. Moreover, gene expression differed betweenGDF8-null animal muscles (tested for nt821del11 and Q204X mutations) and the wild-type muscles for genesGDH1, IGF2R, FADS1, ASPH, andAIM1, all showing down-regulation in Asturiana de los Valles calves with muscle hypertrophy (GDF8-null). This shows that inGDF8-null animals other pathways are used for FA synthesis.

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References

  1. Aldai N, Najera AI, Dugan MER, Celaya R, Osoro K, 2007. Characterisation of intramuscular, intermuscular and subcutaneous adipose tissues in yearling bulls of different genetic groups. Meat Sci 76: 682–691.

  2. Aldai N, Murray BE, Oliván M, Martínez A, Troy DJ, Osoro K, Nájera AI, 2006. The influence of breed and mh-genotype on carcass conformation, meta physico-chemical characteristics, and the fatty acid profile of muscle from yearling bulls. Meat Sci 72: 486–495.

  3. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, 1990. Basic local alignment search tool. J Mol Biol 215:403–410.

  4. Arthur PF, 1995. Double muscling in cattle: a review. Aust J Agric Res 46: 1493–1515.

  5. Bassam BJ, Caetano-Anolles G, Gresshoff PM, 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196: 80–83.

  6. Bernard C, Cassar-Malek I, Le Cunff M, Dubroeucq H, Renand G, Hocquette JF, 2007. New indicators of beef sensory quality revealed by expression of specific genes. J Agric Food Chem 55: 5229–5237.

  7. Boss O, Hagen T, Lowell BB, 2000. Uncoupling proteins 2 and 3: potential regulators of mitochondrial energy metabolism. Diabetes 49:143–156.

  8. Calder PC, 2006. Polyunsaturated fatty acids and inflammation. Prostaglandins Leukot Essent Fatty Acids 75:197–202.

  9. Dannenberger D, Nuernberg G, Scollan N, Ender K, Nuernberg K, 2007. Diet alters the fatty acid composition of individual phospholipid classes in beef muscle. J Agric Food Chem 55; 452–460.

  10. De Smet S, Raes K, Demeyer D, 2004. Meat fatty acid composition as affected by fatness and genetic factors: a review. Anim Res 53: 81–98.

  11. Enser M, Hallett KG, Hewett B, Fursey GAJ, Wood JD, Harrington G, 1998. Fatty acid content and composition of UK beef and lamb muscle in relation to production system and implications for human nutrition. Meat Sci 49: 329–341.

  12. Fagali N, Catalá A, 2007. The effect of melatonin and structural analogues on the lipid peroxidation of triglycerides enriched in omega-3 polyunsaturated fatty acids. Life Sci 81:299–305.

  13. Feriotto G, Finotti A, Breveglieri G, Treves S, Zorzato F, Gambari R, 2006. Multiple levels of control of theexpression of the human A beta H-J-J locus encoding aspartylbeta-hydroxylase, junctin, and junctate. Ann N Y Acad Sci 1091:184–190.

  14. Fernandez X, Monin G, Talmant A, Mourot J, Lebret B, 1999. Influence of intramuscular fat content on the quality of pig meat. 2. Consumer acceptability ofm. longissimus lumborum. Meat Sci 53:67–72.

  15. Fleury C, Neverova M, Collins S, Raimbault S, Champigny O, Levi-Meyrueis C, et al 1997. Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nat Genet 15:269–272.

  16. Laaksonen DE, Nyyssönen K, Niskanen L, Rissanen TH, Salonen JT, 2005. Prediction of cardiovascular mortality in middle-aged men by dietary and serum linoleic and polyunsaturated fatty acids. Arch Intern Med 165: 193–199.

  17. Leaden PJ, Catalá A, 2007. Melatonin and N-acetyl serotonin inhibit selectively enzymatic and non-enzymatic lipid peroxidation of rat liver microsomes. Prostaglandins Leukot Essent Fatty Acids 77:29–35.

  18. Leon J, Acuńa-Castroviejo D, Escames G, Tan DX, Reiter RJ, 2005. Melatonin mitigates mitochondrial malfunction. J Pineal Res 38:1–9.

  19. Lin J, Zhang SM, Cook NCR, Lee LM, Buring JE, 2004. Dietary fat and fatty acids and risk of colorectal cancer in women. Am J Epidemiol 160: 1011–1022.

  20. Liu DH, Yuan HY, Cao CY, Gao ZP, Zhu BY, Huang HL, Liao DF, 2007. Heat shock protein 90 acts as a molecular chaperone in late-phase activation of extracellular signal-regulated kinase 1/2 stimulated by oxidative stress in vascular smooth muscle cells. Acta Pharmacol Sin 28: 1907–1913.

  21. Markham NR, Zuker M, 2005. DINA Melt web server for nucleic acid melting prediction. Nucleic Acids Res 33: W577–581.

  22. Mir PS, McAllister TA, Scott S, Aalhus J, Baron V, McCartney D, et al. 2004. Conjugated linoleic acid-enriched beef production. Am J Clin Nutr 79: 1207S-1211S.

  23. Nezer C, Moreau L, Brouwers B, Coppieters W, Detilleux J, Hanset R, et al. 1999. An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs. Nat Genet 21: 155–156.

  24. Nürnberg K, Wegner J, Ender K, 1998. Factors influencing fat composition in muscle and adipose tissue of farm animals. Livest Prod Sci 56: 145–156.

  25. Ohlund I, Hörnell A, Lind T, Hernell O, 2007. Dietary fat in infancy should be more focussed on quality than on quantity. Eur J Clin Nutr 62: 1058–1064.

  26. Olsvik PA, Lie KK, Jordal AE, Nilsen TO, Hordvik I, 2005. Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon. BMC Mol Biol 6: 21.

  27. Pérez R, Tupac-Yupanqui I, Dunner S, 2008. Evaluation of suitable reference genes for gene expression studies in bovine musculartissue. BMC Mol Biol 9: 79.

  28. Raes K, De Smet S, Demeyer D, 2004. Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review. Anim. Feed Sci. Technol 113: 199–221.

  29. Rozen S, Skaletsky H, 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132: 365–386.

  30. Scollan ND, Choi NJ, Kurt E, Fisher AV, Enser M, Wood JD. (2001). Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. Br J Nutr. 85:115–24.

  31. Scollan ND, Enser M, Gulati SK, Richardson I, Wood JD, 2003. Effects of including a ruminally protected lipid supplement in the diet on the fatty acid composition of beef muscle. Br J Nutr 90: 709–716.

  32. Schaeffer L, Gohlke H, Müller M, Heid IM, Palmer LJ, Kompauer I, et al. 2006. Common genetic variants of the FADS1 FADS2 gene cluster and their reconstructed haplotypes are associated with the fatty acid composition in phospholipids. Hum Mol Genet 15: 1745–1756.

  33. Sherman EL, Nkrumah JD, Murdoch BM, Li C, Wang Z, Fu A, Moore SS, 2008. Polymorphisms and haplotypes in the bovine neuropeptide Y, growth hormonereceptor, ghrelin, insulin-likegrowthfactor 2, and uncoupling proteins 2 and 3 genes and their associations with measures of growth, performance, feed efficiency, and carcass merit in beef cattle. J Anim Sci 86: 1–16.

  34. Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, et al. 1999. Housekeeping genes as internal standards: use and limits. J Biotechnol 75: 291–295.

  35. Treves S, Franzini-Armstrong C, Moccagatta L, Arnoult C, Grasso C, Schrum A et al. 2004. Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion. J Cell Biol 166: 537–548.

  36. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F, 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: research 0034.1- 0034.11. VonSchacky C, 2000. N-3 fatty acids and the prevention of coronary atherosclerosis. Am J Clin Nutr 71: 224S-227S.

  37. Webb EC, O’Neill HA, 2008. The animal fat paradox and meat quality. Meat Sci 80: 28–36.

  38. Wheeler D, Bhagwat M, 2007. BLAST QuickStart: example-driven Web-based BLAST tutorial. Methods Mol Biol 395: 149–176.

  39. Wood JD, Enser M, Fisher AV, Nute GR, Richardson RI, Sheard PR, 1999. Manipulating meat quality and composition. Proc Nutr Soc 58: 363–370.

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Correspondence to S. Dunner.

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Perez, R., Cañón, J. & Dunner, S. Genes associated with long-chain omega-3 fatty acids in bovine skeletal muscle. J Appl Genet 51, 479–487 (2010). https://doi.org/10.1007/BF03208877

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Keywords

  • differential display
  • lipid metabolism
  • long-chain fatty acids
  • meat quality
  • omega-3 fatty acids