Wild pear (Pyrus pyraster, syn.P. communis var.pyraster) is thought to be one of the species that gave rise to all other members of the genusPyrus, although intraspecific hybridizations with cultivated varieties could cause the disappearance of original species characteristics. S-RNase alleles from 7 different wild pear individuals, collected from various regions of Poland, were cloned on the basis of the PCR method and nucleotide sequence analyses. The hypervariable (HV) region is responsible for allele-specific S-RNase activity in the self-incompatibility mechanism. The high level of polymorphism of its sequences may constitute a source of valuable phylogenetic information. From all individuals, 14 sequences were obtained successfully, and 9 of them were novel alleles. Phylogenetic analysis of these alleles was based on the amino acid sequence interpretation of coding regions and intron nucleotide sequences. The research conducted on a limited pool of availableP. pyraster alleles gives only an initial insight into possible S-RNase allele polymorphisms in wild populations. At this stage, the results do not confirm a strong influence of cultivated pear species on the wild pear.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Dolatowski JNJ, Podyma W, Szymańska M, Zych M, 2004. Molecular studies on the variability of Polish semi-wildpears (Pyrus) using AFLP. Journal of Fruit and Ornamental Plant Research, Special edn. 12: 331–337.
Dostalek J, 1989.Pyrus × amphigenea, seine Taxonomic und Nomenklatur. Folia Goeobot. & Phytotaxonomica 24: 103–108.
Holderegger R, Häner R, Csencsics D, Angelone S, Hoebee SE, 2008. S-allele diversity suggests nomate limitation in small populations of a self-incompatible plant. Evolution 62: 2922–2928.
Ishimizu T, Inoue K, Shimonaka M, Saito T, Terai O, Norioka S, 1999. PCR-based method for identifying the S-genotypes of Japanese pear cultivars. Theor. Appl. Genet. 98: 961–967.
Kim HT, Hirata Y, Nou IS, 2002. Determination of S-genotypes of pear (Pyrus pyrifolia) cultivars by S-RNase sequencing and PCR-RFLP analyses. Mol Cells. 13: 444–451.
Kim HT, HirataY, Kim HJ, Nou IS, 2006. The presence of a new S-RNase Allele (S10) in Asian pear (Pyrus pyrifolia (Burm; Nakai). Genet. Resour. Crop Evol. 53: 1374–1383.
Ma RC, Oliveira MM, 2002. Evolutionary analysis of S-RNase genes from Rosaceae species. Mol Genet Genomics 267: 71–78.
McClure BA, Franklin-Tong VE, 2006. Gametophytic self-incompatibility: understanding the cellular mechanisms involved in “self” pollen tube inhibition. Planta 224: 233–245.
Palombi MA, Lombardo B, Caboni E, 2007. In vitro regeneration of wild pear (Pyrus pyraster Burgsd) clones tolerant to Fe-chlorosis and somaclonal variation analysis by RAPD markers. Plant Cell Rep. 26: 489–496.
Richman AD, Kohn JR, 2000. Evolutionary genetics of self-incompatibility in the Solanaceae. Plant Mol. Biol. 42: 169–179.
Stephan BR, Wagner I, Keinschmit J, 2003. Euroforgen technical guidelines for genetic conservation anduse for wild apple and pear (Malus sylvestris andPyrus pyraster). IPGRI. Euroforgen Noble Hardwoods Networks.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV, 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531–6535.
About this article
Cite this article
Wolko, Ł., Antkowiak, W., Sips, M. et al. Self-incompatibility alleles in Polish wild pear (Pyrus pyraster (L.) Burgsd.): a preliminary analysis. J Appl Genet 51, 33–35 (2010). https://doi.org/10.1007/BF03195708
- Pyrus pyraster