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Role ofADRB2 gene polymorphism in asthma and response to β2-agonists in Polish children

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Abstract

The aims of this study were: (1) to find associations of asthma with single-nucleotide polymorphisms (SNPs) within theADRB2 gene: Arg16Gly, Gln27Glu, −1023 G/A, −367 T/C, −47 C/T ; (2) to define linkage disequilibrium in the gene region, basing on the analyzed SNPs; and (3) to analyze the importance ofADRB2 polymorphism for response to bronchodilator drugs in children diagnosed with bronchial asthma. We compared 113 asthmatic children and 123 healthy subjects from the Polish population. Genotyping was performed by PCR-RFLP. We found an association of the A allele of −1023A/GADRB2 polymorphism with asthma (P = 0.024). No significant associations with other SNPs were detected. Moderate linkage was found between Gln27Glu and −47C/T polymorphisms in linkage disequilibrium analysis (D’ = 0.85,r 2 = 0.429, LOD = 31.97). No significant differences were found in haplotype frequencies in comparison to the control group, implicating that they are not associated with susceptibility to asthma in the analyzed population. There was no significant correlation between the analyzed SNPs of theADRB2 gene and the response to β2-agonists. This is the first report providing suggestive evidence for association of —1023A/GADRB2 polymorphism with an increased risk of asthma. The analyzed SNPs may not play a major role in response to β2-agonists in asthmatic children.

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Correspondence to A. Szczepankiewicz.

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Szczepankiewicz, A., Bręborowicz, A., Sobkowiak, P. et al. Role ofADRB2 gene polymorphism in asthma and response to β2-agonists in Polish children. J Appl Genet 50, 275–281 (2009). https://doi.org/10.1007/BF03195683

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Keywords

  • association
  • asthma
  • β2-adrenergic receptor gene
  • β2-adrenoceptor gene
  • linkage disequilibrium
  • pharmacogenetics
  • polymorphism