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Journal of Applied Genetics

, Volume 49, Issue 4, pp 433–441 | Cite as

Wheat-infectingFusarium species in Poland — their chemotypes and frequencies revealed by PCR assay

  • Łukasz StępieńEmail author
  • Delfina Popiel
  • Grzegorz Koczyk
  • Jerzy Chełkowski
Original Article

Abstract

ThreeFusarium species:F. graminearum, F. culmorum andF. cerealis were identified in laboratory cultures and in sporodochia from spikelets of scabby wheat. SCAR (sequence characterized amplified region) primers were used to identifyFusarium species and nivalenol (NIV) and deoxynivalenol (DON) chemotypes within species in laboratory cultures and field collected heads harvested in 2006. Results from PCR analyses confirmed preliminary identifications of species on the basis of examination of macroconidia under a light microscope and identification of cultures on agar media. NIV and DON (3Ac-DON and 15Ac-DON) chemotypes were identified using PCR assay. Among samples and isolates ofF. graminearum, the 15Ac-DON chemotype dominated, and among those whereF. culmorum was identified, the 3Ac-DON chemotype prevailed. Only 5 of the 41 isolates ofF. graminearum tested, displayed the NIV chemotype. An increase in the frequency ofF. graminearum and a decrease in the frequency ofF. culmorum were found during 1998 to 2006.

Keywords

deoxynivalenol Fusarium species nivalenol wheat head blight chemotypes SCARs 

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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2008

Authors and Affiliations

  • Łukasz Stępień
    • 1
    • 2
    Email author
  • Delfina Popiel
    • 1
  • Grzegorz Koczyk
    • 1
  • Jerzy Chełkowski
    • 1
  1. 1.Institute of Plant GeneticsPolish Academy of SciencesPoznańPoland
  2. 2.Department of Molecular Biology, Institute of Plant GeneticsPolish Academy of SciencesPoznańPoland

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