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Cross-reactivity of antibodies against T-2 with deepoxide T-2 toxin

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Abstract

Two types of antibodies raised against T-2 toxin, namely anti-T-2-HS-BSA and anti-3 -Ac -NEOS-HS -BSA, showed good cross-reactivity with deepoxy T-2 toxin. Our results indicate that the epoxide is not an important epitope for the production of antibody against T-2 toxin

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References

  1. 1

    Bamburg JR, Strong FM (1971) 12,13-Epoxytrichothecenes. In: Kadis S, Ciegler A, Ajl SJ (eds) Microbial Toxins, Vol 7. Algal and Fungal Toxins. Academic Press, Inc., New York, pp 207–292

  2. 2

    Ellison RA, Kotsonis FN (1974)In vitro metabolism of T-2 toxin. Appl Microbiol 27:423–424

  3. 3

    Knupp CA, Swanson SP, Buck WB (1976)In vitro metabolism of T-2 toxin by rat liver microsomes. J Agric Food Chem 34:865–868

  4. 4

    Ohta M, Ishii K, Ueno Y (1977) Metabolism of trichothecene mycotoxins. I. Microsomal deacetylation of T-2 toxin in animal tissues. J Biochem 82:1591–1598

  5. 5

    Visconti A, Mirocha CJ (1985) Identification of various T-2 toxin metabolites in chicken excreta and tissues. Appl Environ Microbiol 49:1246–1250

  6. 6

    Yoshizawa T, Mirocha CJ, Behrens JC, Swanson SP (1981) Metabolic fate of T-2 toxin in a lactating cow. Fd Cosmet toxicol 19:31–39

  7. 7

    Wei RD, Chu FS (1985) Modification ofin vitro metabolism of T-2 toxin by esterase inhibitors. Appl Environ Microbiol 50:115 -119

  8. 8

    Yoshizawa T, Sakamoto T, Ayano Y, Mirocha CJ (1982) 3′-Hydroxy T-2 and 3′-hydroxy HT-2 toxin: New metabolites of T-2 toxin, a trichothecene mycotoxin, in animals. Agric Biol Chem 46:2613–2515

  9. 9

    Yoshizawa T, Sakamoto T, Okamoto K (1984)In vitro formation of 3′-hydroxy T-2 and 3′-hydroxy HT-2 toxins from T-2 toxin by liver homogenates from mice and monkeys. Appl Environ Microbiol 47:130–134

  10. 10

    Corley RA, Swanson SP, Buck WB (1985) Glucuronide conjugates of T-2 toxin and metabolites in swine bile and urine. J Agric Food Chem 33:1085–1089

  11. 11

    Swanson SP, Nicolletti J, Rood HD, Buck WB, Cote LM, Yoshizawa T (1987) Metabolism of three trichothecene mycotoxins, T-2 toxin, diacetoxyscirpenol and deoxynivalenol, by bovine rumen microorganisms. J Chromatogr 414: 335–342

  12. 12

    Yoshizawa T, Cote LM, Swanson SP, Buck WB (1986) Confirmation of DOM-1, a deepoxidation metabolite of deoxynivalenol, in biological fluids of lactating cows. Agric Biol Chem 50:227–229

  13. 13

    Yoshizawa T, Sakamoto T, Kuwamura K (1985) Structures of deepoxytrichothecene metabolites from 3′-hydroxy HT-2 toxin and T-2 tetraol in rats. Appl Environ Microbiol 50:676–679

  14. 14

    Chu FS, Grossman S, Wei RD, Mirocha CJ (1979) Production of antibody against T-2 toxin. Appl Environ Microbiol 37:104–108

  15. 15

    Fan TSL, Zhang GS, Chu FS (1987) Production and characterization of antibodies against HT-2 toxin and T-2 tetraol tetraacetate. Appl Environ Microbiol 53:17–21

  16. 16

    Hunter KW, Brimfield AA, Miller M, Finkelman FD, Chu FS (1985) Preparation and characterization of monoclonal antibodies to the trichothecene mycotoxin T-2. Appl Environ Microbiol 49:168–172

  17. 17

    Wei RD, Bischoff W, Chu FS (1986) Production and characterization of antibody against 3′-OH-T-2 toxin. J Food Protection 49:267–271

  18. 18

    Wei RD, Chu FS (1987) Production and characterization of a generic antibody against group A trichothecenes. Anal Biochem 160:399–408

  19. 19

    Zhang GS, Schubring SL, Chu FS (1986) Improved method for production of antibodies against T-2 toxin and diacetoxyscirpenol in rabbits. Appl Environ Microbiol 51:132–137

  20. 20

    Chu FS (1985) Recent studies on immunochemical analysis of mycotoxins. In: Steyn PS, Vleggaar R (eds) Mycotoxins and Phycotoxins. Elsevier Science Publishers, Amsterdam, pp 277–292

  21. 21

    Wei RD, Strong FM, Smalley EB, Schnoes HK (1971) Chemical interconversion of T-2 and HT-2 toxins and related compounds. Biochem Biophys Res Commun 45:396–401

  22. 22

    Wallace EM, Pathre SV, Mirocha CJ, Robison TS, Fenton SWF (1977) Synthesis of radiolabeled T-2 toxin. J Agric Food Chem 25:836–838

  23. 23

    Colvin EW, Cameron SW (1986) Chemical deoxygenation of the trichothecenes, diacetoxyscirpenol and deoxynivalenol. J Chem Soc, Chem Comm 467:1084–1085

  24. 24

    Sigg HP, Mauli R, Flury E, Hauser D (1965) Die Konstitution von Diacetoxyscirpenol. Helv Chem Acta 48:962–988

  25. 25

    Hewetson JF, Pace JG, Beheler JA (1987) Detection and Quantitation of T-2 mycotoxin in rat organs by radioimmunoassay. J Assoc Off Anal Chem 70:654–657

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Wei, R., Swanson, S.P. & Chu, F.S. Cross-reactivity of antibodies against T-2 with deepoxide T-2 toxin. Mycotox Res 4, 15–19 (1988). https://doi.org/10.1007/BF03192084

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Keywords

  • Trichothecene
  • Deoxynivalenol
  • Mycotoxin Research
  • Trichothecene Mycotoxin
  • Tetraol