Mycotoxin Research

, Volume 12, Issue 1, pp 7–13 | Cite as

Production of14C-ochratoxin A byPenicillium verrucosum sp. 1761 in liquid culture

  • M Ruhland
  • G Engelhardt
  • P R Wallnöter
Article
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Summary

The synthesis of14C-labelled ochratoxin A was carried out by biosynthesis withPénicillium verrucosum sp. 1761 in liquid culture using 2-14C-Na-acetate and 2-14C-malonic acid as precursor. The largest amount of ochratoxin A produced byPénicillium verrucosum was detected in a minimal medium after an incubation period of 28 days. By addition of citric acid and 2,4-dichlorophenoxy acetic acid larger ochratoxin A contents could be measured. For transformation studies of ochratoxin A, a molecule which is exclusively labelled in the isocoumarin moiety is best suitable. This labelling could be obtained by using labelled malonic acid as precursor, whereas with 2-14C-Na-acetate as precursor the whole molecule was labelled. Incorporation rates of radioactivity into the molecule were from 0.04% to 0.11%, specific radioactivities from 0.02 μCi/mg to 0.07 μCi/mg.

Keywords

Citric Acid Aflatoxin Malonic Acid Incorporation Rate Mycotoxin Research 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Literature

  1. 1.
    Aydes J, Mateles RI, 1964.Incorporation of labelled compounds into aflatoxins. Biochim Biophys Acta 86, 418–420Google Scholar
  2. 2.
    Bacon CW, Robbins JD, Burdick D, 1975. Metabolism of glutamic acid inAspergillus ochraceus during the biosynthesis of ochratoxin A. Appl Microbiol 29, 317–322PubMedGoogle Scholar
  3. 3.
    Ciegler A, 1972. Bioproduction of ochratoxin A and penicillic acid by members of the,Aspergillus ochraceus group. Can J Microbiol 18, 631–636CrossRefPubMedGoogle Scholar
  4. 4.
    Cohen H, Lapointe M, 1986.Determination of ochratoxin A in animal feed and cereal grains by liquid chromatography with fluorescence detection. J Ass Off Anal Chem 69, 957–959Google Scholar
  5. 5.
    El-Shayeb NMA, Mabrouck SS, Abd-El-Fattah AMM, 1992. Production of ochratoxins by some egyptianAspergillus strains. Zentralbl Mikrobiol 147, 86–91PubMedGoogle Scholar
  6. 6.
    Kraus GA, 1981. A facile synthesis of ochratoxin A. J Org Chem 46, 201–202CrossRefGoogle Scholar
  7. 7.
    Königer M, Süß A, 1968. The use of gelating agents in assaying substances labelled with C14. Kerntechnik, Isotopentechnik und Chemie 5, 274–278Google Scholar
  8. 8.
    Lillehoj EB, Aalund O, Haid B, 1978. Bioproduction of14C-ochratoxin A in submerged culture. Appl Environ Microbiol 3, 720–723Google Scholar
  9. 9.
    Pitout MJ, 1969. The hydrolysis of ochratoxin A by some proteolytic enzymes. Biochem Pharmacol. 18, 485–491CrossRefPubMedGoogle Scholar
  10. 10.
    Pohland AE, Nesheim S, Frieman L, 1992. Ochratoxin A: A review. Pure Appl Chem 64, 1029–1046CrossRefGoogle Scholar
  11. 11.
    Roberts JC, Woolven P, 1970. Studies in mycological chemistry. Part XXIV. Synthesis of ochratoxin A, a metabolite ofAspergillus ochraceus Wilh. J Chem Soc C, 278–281Google Scholar
  12. 12.
    Sansing GA, Davies ND, Diener UL, 1973. Effect of time and temperature on ochratoxin A production byAspergillus ochmceus. Can J Microbiol 19, 1259–1263CrossRefPubMedGoogle Scholar
  13. 13.
    Searcy JW, Davies ND, Diener UL, 1969. Biosynthesis of ochratoxin A. Appl Microbiol 18, 622–627PubMedGoogle Scholar
  14. 14.
    Steyn PS, Holzapfel CW, 1967. The synthesis of ochratoxin A and B, metabolites ofAspergillus ochraceus Wilh. Tetrahedron 23, 4459–4461CrossRefGoogle Scholar
  15. 15.
    Van der Merwe KJ, Steyn PS, Fourie L, 1965. Mycotoxins. Part II. The constitution of ochratoxin A, B, and C, metabolites ofAspergillus ochraceus Wilh. J Chem Soc 7083–7088Google Scholar
  16. 16.
    Yamazaki M, Maebayashi Y, Miyaki K, 1970. Production of ochratoxin A byAspergillus ochraceus isolated in Japan from moldy rice. Appl Microbiol 20, 452–454PubMedGoogle Scholar

Copyright information

© Society of Mycotoxin Research and Springer 1996

Authors and Affiliations

  • M Ruhland
    • 1
  • G Engelhardt
    • 1
  • P R Wallnöter
    • 1
  1. 1.Bayerische Landesanstalt für ErnährungMünchenGermany

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