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On the metabolism of the mycotoxin deoxynivalenol in the isolated perfused rat liver

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An isolated rat liver was perfused with deoxynivalenol (DON) at a dose of 3 mg in a recirculating perfusion system. To identify glucuronide conjugates equal amounts of bile samples, perfusate and liver homogenates were incubated with and without (control) a β-glucuronidase preparation and analyzed by thin layer chromatography and capillary gas liquid chromatography — chemical ionization mass spectrometry. A total of 40.4% of the administered dose of DON was found to be conjugated with glucuronic acid (perfusate 20.4%, bile 19.2%, liver 0.8%), while only 1.3% of the parent DON (perfusate 1.1%, bile 0.2%) was detected. The cleavage of DON-glucuronide was demonstrated by incubating DON-glucuronide containing bile samples with intestine contents under anaerobic conditions.

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Correspondence to M Gareis.

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Gareis, M., Bauer, J. & Gedek, B. On the metabolism of the mycotoxin deoxynivalenol in the isolated perfused rat liver. Mycotox Res 3, 25–32 (1987). https://doi.org/10.1007/BF03191982

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  • Trichothecene
  • Deoxynivalenol
  • Mycotoxin Research
  • Bile Sample
  • Trichothecene Mycotoxin